ArticleScientific reports2016
Transgenic Eimeria tenella as a vaccine vehicle: expressing TgSAG1 elicits protective immunity against Toxoplasma gondii infections in chickens and mice.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- An oral Eimeria-vectored vaccine induces protective immunity against chicken infectious anemia.NPJ vaccines · 2026Article
- Precocious Eimeria magna transgenically expressing RHDV P2 subdomain induces immune responses in rabbits.NPJ vaccines · 2025Article
- Genetic manipulation for the non-model protozoaniScience · 2025Review
- PLGA nanoparticles as an efficient carrier inFrontiers in immunology · 2025Article
- Developing efficient strategies for localizing the enhanced yellow fluorescent protein subcellularly in transgenic Eimeria parasites.Scientific reports · 2024Article
- Microneme-located VP2 inInfection and immunity · 2024Article
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- Vaccines against chicken coccidiosis with particular reference to previous decade: progress, challenges, and opportunities.Parasitology research · 2022Review
- Nano vaccines forFrontiers in immunology · 2022Article
- Establishment of RecombinantVaccines · 2021Article
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- RecombinantVaccines · 2021Article
- With Chitosan and PLGA as the Delivery Vehicle,Frontiers in immunology · 2021Article
- Modulation Effects ofVaccines · 2020Article
- Epidemiologic significance ofParasitology · 2020Review
- Towards Innovative Design and Application of RecombinantInfection and immunity · 2020Review
- Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection.Parasites & vectors · 2019Article
- Transgenic Eimeria tenella Expressing Profilin of Eimeria maxima Elicits Enhanced Protective Immunity and Alters Gut Microbiome of Chickens.Infection and immunity · 2018Article
- Mutual interactions of the apicomplexan parasites Toxoplasma gondii and Eimeria tenella with cultured poultry macrophages.Parasites & vectors · 2018Article
- Chitosan Microsphere Used as an Effective System to Deliver a Linked Antigenic Peptides Vaccine Protect Mice Against Acute and ChronicFrontiers in cellular and infection microbiology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The surface antigen 1 of Toxoplasma gondii (TgSAG1) is a major immunodominant antigen and is widely considered an ideal candidate for the development of an effective recombinant vaccine against toxoplasmosis. Eimeria tenella, an affinis apicomplexan parasite with T. gondii, is a potential vaccine vector carrying exogenous antigens that stimulates specific immune responses. Here, we engineered TgSAG1 into E. tenella and obtained a stably transfected E. tenella line (Et-TgSAG1). We found TgSAG1 localized on the cell surface of Et-TgSAG1, which is similar to its native distribution in T. gondii tachyzoites. We immunized the chickens with Et-TgSAG1 orally and detected TgSAG1-specific immune responses, which partly reduced T. gondii infection. In the mouse model, we immunized the mice with Et-TgSAG1 sporozoites intraperitoneally and challenged them with T. gondii tachyzoites RH strain. We found that the mice immunized with Et-TgSAG1 showed a TgSAG1 specific Th 1-dominant immune response and a prolonged survival time compared with wild-type E. tenella and non-immunized mice. Collectively, our results demonstrated that Et-TgSAG1, utilized as a recombinant vaccine against toxoplasmosis, could be applied in both chickens and mice. Our findings also provide a promising persuasion for the development of transgenic Eimeria as vaccine vectors for use in birds and mammals.
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