ArticleNucleic acids research2016
The mechanism of ϕC31 integrase directionality: experimental analysis and computational modelling.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 23 citations in OpenAlex.
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- Mitigating genetic instability caused by the excision activity of theApplied and environmental microbiology · 2025Article
- A phage weaponizes a satellite recombinase to subvert viral restriction.Nucleic acids research · 2022Article
- Model-guided engineering of DNA sequences with predictable site-specific recombination rates.Nature communications · 2022Article
- A bipartite thermodynamic-kinetic contribution by an activating mutation to RDF-independent excision by a phage serine integrase.Nucleic acids research · 2020Article
- A memory switch for plant synthetic biology based on the phage ϕC31 integration system.Nucleic acids research · 2020Article
- A single-input binary counting module based on serine integrase site-specific recombination.Nucleic acids research · 2019Article
- Two convergent pathways of DNA knotting in replicating DNA molecules as revealed by θ-curve analysis.Nucleic acids research · 2018Article
- Mathematical model of a serine integrase-controlled toggle switch with a single input.Journal of the Royal Society, Interface · 2018Article
- A novel family of tyrosine integrases encoded by the temperate pleolipovirus SNJ2.Nucleic acids research · 2018Article
- A novel non prophage(-like) gene-intervening element within gerE that is reconstituted during sporulation in Bacillus cereus ATCC10987.Scientific reports · 2017Article
- Control of serine integrase recombination directionality by fusion with the directionality factor.Nucleic acids research · 2017Article
- Coiled-coil interactions mediate serine integrase directionality.Nucleic acids research · 2017Article
- Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis.Nucleic acids research · 2017Article
- Control of Recombination Directionality by the Listeria Phage A118 Protein Gp44 and the Coiled-Coil Motif of Its Serine Integrase.Journal of bacteriology · 2017Article
- A simplified mathematical model of directional DNA site-specific recombination by serine integrases.Journal of the Royal Society, Interface · 2017Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Serine integrases, DNA site-specific recombinases used by bacteriophages for integration and excision of their DNA to and from their host genomes, are increasingly being used as tools for programmed rearrangements of DNA molecules for biotechnology and synthetic biology. A useful feature of serine integrases is the simple regulation and unidirectionality of their reactions. Recombination between the phage attP and host attB sites is promoted by the serine integrase alone, giving recombinant attL and attR sites, whereas the 'reverse' reaction (between attL and attR) requires an additional protein, the recombination directionality factor (RDF). Here, we present new experimental data on the kinetics and regulation of recombination reactions mediated by ϕC31 integrase and its RDF, and use these data as the basis for a mathematical model of the reactions. The model accounts for the unidirectionality of the attP × attB and attL × attR reactions by hypothesizing the formation of structurally distinct, kinetically stable integrase-DNA product complexes, dependent on the presence or absence of RDF. The model accounts for all the available experimental data, and predicts how mutations of the proteins or alterations of reaction conditions might increase the conversion efficiency of recombination.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.