ArticleScientific reports2016
CpsR, a GntR family regulator, transcriptionally regulates capsular polysaccharide biosynthesis and governs bacterial virulence in Streptococcus pneumoniae.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Trial
- Transcriptional regulation of the pneumococcal capsule can dictate serotype-specific infection.Nature communications · 2026Article
- Glucose affects capsular polysaccharides synthesis via CcpA and HPr in Streptococcus pneumoniae.Journal of microbiology (Seoul, Korea) · 2025Article
- SPD_0410 negatively regulates capsule polysaccharide synthesis and virulence inFrontiers in microbiology · 2024Article
- Synthetic genetic oscillators demonstrate the functional importance of phenotypic variation in pneumococcal-host interactions.Nature communications · 2023Article
- Effects of Capsular Polysaccharide amount on Pneumococcal-Host interactions.PLoS pathogens · 2023Article
- StkP- and PhpP-Mediated Posttranslational Modifications Modulate the S. pneumoniae Metabolism, Polysaccharide Capsule, and Virulence.Infection and immunity · 2023Article
- Transcriptomics in serum and culture medium reveal shared and differential gene regulation in pathogenic and commensalMicrobial genomics · 2023Article
- Phosphorylation of GntR reduces Streptococcus suis oxidative stress resistance and virulence by inhibiting NADH oxidase transcription.PLoS pathogens · 2023Article
- Pneumococcal capsule expression is controlled through a conserved, distal cis-regulatory element during infection.PLoS pathogens · 2023Article
- Encapsulation of the septal cell wall protects Streptococcus pneumoniae from its major peptidoglycan hydrolase and host defenses.PLoS pathogens · 2022Article
- MgaVirulence · 2021Article
- Article
- Regulation and distinct physiological roles of manganese in bacteria.FEMS microbiology reviews · 2021Review
- Examining the Distribution and Impact of Single-Nucleotide Polymorphisms in the Capsular Locus of Streptococcus pneumoniae Serotype 19A.Infection and immunity · 2021Article
- Article
- The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression.Microorganisms · 2021Article
- Article
- Article
- Identification of a GntR family regulator BusRExtremophiles : life under extreme conditions · 2019Article
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Authors and funding
6 authors.
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Abstract
Transcriptional regulation of capsule expression is critical for pneumococcal transition from carriage to infection, yet the underlying mechanism remains incompletely understood. Here, we describe the regulation of capsular polysaccharide, one of the most important pneumococcal virulence factor by a GntR family regulator, CpsR. Electrophoretic mobility-shift assays have shown the direct interaction between CpsR and the cps promoter (cpsp), and their interaction could be competitively interfered by glucose. DNase I footprinting assays localized the binding site to a region -146 to -114 base pairs relative to the transcriptional start site of the cps locus in S. pneumoniae D39. We found that CpsR negatively controlled the transcription of the cps locus and hence CPS production, which was confirmed by fine-tuning expression of CpsR in a ΔcpsR complemented strain. Increased expression of CpsR in complemented strain led to a decreased resistance to the whole-blood-mediated killing, suggesting a protective role for CpsR-cpsp interaction in the establishment of invasive infection. Finally, animal experiments showed that CpsR-cpsp interaction was necessary for both pneumococcal colonization and invasive infection. Taken together, our results provide a thorough insight into the regulation of capsule production mediated by CpsR and its important roles in pneumococcal pathogenesis.
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