ArticleScience advances2016
Time-gated FRET nanoassemblies for rapid and sensitive intra- and extracellular fluorescence imaging.
Article in Science advances, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 66 citations in OpenAlex.
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- A Nanobody-on-Quantum Dot Displacement Assay for Rapid and Sensitive Quantification of the Epidermal Growth Factor Receptor (EGFR).Angewandte Chemie (International ed. in English) · 2022Article
- Stimulus-responsive room temperature phosphorescence materials with full-color tunability from pure organic amorphous polymers.Science advances · 2022Article
- Completely aqueous processable stimulus responsive organic room temperature phosphorescence materials with tunable afterglow color.Nature communications · 2022Article
- Understanding Förster Resonance Energy Transfer in the Sheet Regime with DNA Brick-Based Dye Networks.ACS nano · 2021Article
- Methods for Intracellular Delivery of Quantum Dots.Topics in current chemistry (Cham) · 2021Review
- New 1,3-Disubstituted Benzo[Molecules (Basel, Switzerland) · 2020Article
- Single-Chain Lanthanide Luminescence Biosensors for Cell-Based Imaging and Screening of Protein-Protein Interactions.iScience · 2020Article
- Lanthanide-Based Optical Probes of Biological Systems.Cell chemical biology · 2020Review
- In Vivo Biosensing Using Resonance Energy Transfer.Biosensors · 2019Review
- Time-Gated Luminescence Detection of Enzymatically Produced Hydrogen Sulfide: Design, Synthesis, and Application of a Lanthanide-Based Probe.Inorganic chemistry · 2018Article
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 3 countries.
Funding
Abstract
Time-gated Förster resonance energy transfer (FRET) using the unique material combination of long-lifetime terbium complexes (Tb) and semiconductor quantum dots (QDs) provides many advantages for highly sensitive and multiplexed biosensing. Although time-gated detection can efficiently suppress sample autofluorescence and background fluorescence from directly excited FRET acceptors, Tb-to-QD FRET has rarely been exploited for biomolecular imaging. We demonstrate Tb-to-QD time-gated FRET nanoassemblies that can be applied for intra- and extracellular imaging. Immunostaining of different epitopes of the epidermal growth factor receptor (EGFR) with Tb- and QD-conjugated antibodies and nanobodies allowed for efficient Tb-to-QD FRET on A431 cell membranes. The broad usability of Tb-to-QD FRET was further demonstrated by intracellular Tb-to-QD FRET and Tb-to-QD-to-dye FRET using microinjection as well as cell-penetrating peptide-mediated endocytosis with HeLa cells. Effective brightness enhancement by FRET from several Tb to the same QD, the use of low nanomolar concentrations, and the quick and sensitive detection void of FRET acceptor background fluorescence are important advantages for advanced intra- and extracellular imaging of biomolecular interactions.
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Registered trials
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