ArticleAdipocyte
An siRNA-based method for efficient silencing of gene expression in mature brown adipocytes.
Article in Adipocyte. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 55 citations in OpenAlex.
- Article
- RBM43 controls PGC1α translation and a PGC1α-STING signaling axis.Cell metabolism · 2025Article
- Transcriptomic signatures of cold acclimated adipocytes reveal CXCL12 as a Brown autocrine and paracrine chemokine.Molecular metabolism · 2025Article
- LGR signaling mediates muscle-adipose tissue crosstalk and protects against diet-induced insulin resistance.Nature communications · 2024Article
- Monocarboxylate transporters facilitate succinate uptake into brown adipocytes.Nature metabolism · 2024Article
- An optimized method for gene knockdown in differentiating human and mouse adipocyte cultures.bioRxiv : the preprint server for biology · 2023Article
- Quantitative analysis of metabolic fluxes in brown fat and skeletal muscle during thermogenesis.Nature metabolism · 2023Article
- Integrative genomic analyses in adipocytes implicate DNA methylation in human obesity and diabetes.Nature communications · 2023Article
- Myoglobin-mediated lipid shuttling increases adrenergic activation of brown and white adipocyte metabolism and is as a marker of thermogenic adipocytes in humans.Clinical and translational medicine · 2022Article
- Architecture of the outbred brown fat proteome defines regulators of metabolic physiology.Cell · 2022Article
- Immunoglobulin Superfamily Containing Leucine-Rich Repeat (Islr) Participates in IL-6-Mediated Crosstalk between Muscle and Brown Adipose Tissue to Regulate Energy Homeostasis.International journal of molecular sciences · 2022Article
- Insulin resistance rewires the metabolic gene program and glucose utilization in human white adipocytes.International journal of obesity (2005) · 2022Article
- The integrin beta1 modulator Tirofiban prevents adipogenesis and obesity by the overexpression of integrin-linked kinase: a pre-clinical approach in vitro and in vivo.Cell & bioscience · 2022Article
- An adipose lncRAP2-Igf2bp2 complex enhances adipogenesis and energy expenditure by stabilizing target mRNAs.iScience · 2022Article
- Adipose MDM2 regulates systemic insulin sensitivity.Scientific reports · 2021Article
- Therapeutic Perspectives of Thermogenic Adipocytes in Obesity and Related Complications.International journal of molecular sciences · 2021Review
- Article
- Asc-1 regulates white versus beige adipocyte fate in a subcutaneous stromal cell population.Nature communications · 2021Article
- MiR-22 modulates brown adipocyte thermogenesis by synergistically activating the glycolytic and mTORC1 signaling pathways.Theranostics · 2021Article
- Isolation, Culture, and Functional Analysis of Murine Thermogenic Adipocytes.STAR protocols · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brown adipose tissue is a promising therapeutic target for opposing obesity, glucose intolerance and insulin resistance. The ability to modulate gene expression in mature brown adipocytes is important to understand brown adipocyte function and delineate novel regulatory mechanisms of non-shivering thermogenesis. The aim of this study was to optimize a lipofection-based small interfering RNA (siRNA) transfection protocol for efficient silencing of gene expression in mature brown adipocytes. We determined that a critical parameter was to deliver the siRNA to mature adipocytes by reverse transfection, i.e. transfection of non-adherent cells. Using this protocol, we effectively knocked down both high- and low-abundance transcripts in a model of mature brown adipocytes (WT-1) as well as in primary mature mouse brown adipocytes. A functional consequence of the knockdown was confirmed by an attenuated increase in uncoupled respiration (thermogenesis) in response to β-adrenergic stimulation of mature WT-1 brown adipocytes transfected with uncoupling protein 1 siRNA. Efficient gene silencing was also obtained in various mouse and human white adipocyte models (3T3-L1, primary mouse white adipocytes, hMADS) with the ability to undergo "browning." In summary, we report an easy and versatile reverse siRNA transfection protocol to achieve specific silencing of gene expression in various models of mature brown and browning-competent white adipocytes, including primary cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.