ArticleJournal of neurophysiology2016
Integration of autopatching with automated pipette and cell detection in vitro.
Article in Journal of neurophysiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Inwardly rectifying potassium channels promote directional sensing during neutrophil chemotaxis.The Journal of cell biology · 2026Article
- Computer vision-guided rapid and precise automated cranial microsurgeries in mice.Science advances · 2025Article
- Inwardly rectifying potassium channels regulate membrane potential polarization and direction sensing during neutrophil chemotaxis.bioRxiv : the preprint server for biology · 2025Article
- Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections.eLife · 2024Article
- Patch-walking: Coordinated multi-pipette patch clamp for efficiently finding synaptic connections.bioRxiv : the preprint server for biology · 2024Article
- High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot.Genetics · 2024Article
- Neuron Contact Detection Based on Pipette Precise Positioning for Robotic Brain-Slice Patch Clamps.Sensors (Basel, Switzerland) · 2023Article
- Multiscale, multi-perspective imaging assisted robotic microinjection of 3D biological structures.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2021Article
- Automated microscope-independent fluorescence-guided micropipette.Biomedical optics express · 2021Article
- Visual Familiarity Induced 5-Hz Oscillations and Improved Orientation and Direction Selectivities in V1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Deep learning-based real-time detection of neurons in brain slices for in vitro physiology.Scientific reports · 2021Article
- Automatic deep learning-driven label-free image-guided patch clamp system.Nature communications · 2021Article
- Single neuron recording: progress towards high-throughput analysis.Bioelectronics in medicine · 2020Article
- Fabrication andJournal of electroanalytical chemistry (Lausanne, Switzerland) · 2020Article
- Visual Experience-Dependent Oscillations and Underlying Circuit Connectivity Changes Are Impaired in Fmr1 KO Mice.Cell reports · 2020Article
- Vision-Based Sensor for Three-Dimensional Vibrational Motion Detection in Biological Cell Injection.Sensors (Basel, Switzerland) · 2019Article
- Article
- Robotic platform for microinjection into single cells in brain tissue.EMBO reports · 2019Article
- PatcherBot: a single-cell electrophysiology robot for adherent cells and brain slices.Journal of neural engineering · 2019Article
- Accurate signal-source localization in brain slices by means of high-density microelectrode arrays.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Patch clamp is the main technique for measuring electrical properties of individual cells. Since its discovery in 1976 by Neher and Sakmann, patch clamp has been instrumental in broadening our understanding of the fundamental properties of ion channels and synapses in neurons. The conventional patch-clamp method requires manual, precise positioning of a glass micropipette against the cell membrane of a visually identified target neuron. Subsequently, a tight "gigaseal" connection between the pipette and the cell membrane is established, and suction is applied to establish the whole cell patch configuration to perform electrophysiological recordings. This procedure is repeated manually for each individual cell, making it labor intensive and time consuming. In this article we describe the development of a new automatic patch-clamp system for brain slices, which integrates all steps of the patch-clamp process: image acquisition through a microscope, computer vision-based identification of a patch pipette and fluorescently labeled neurons, micromanipulator control, and automated patching. We validated our system in brain slices from wild-type and transgenic mice expressing channelrhodopsin 2 under the Thy1 promoter (line 18) or injected with a herpes simplex virus-expressing archaerhodopsin, ArchT. Our computer vision-based algorithm makes the fluorescent cell detection and targeting user independent. Compared with manual patching, our system is superior in both success rate and average trial duration. It provides more reliable trial-to-trial control of the patching process and improves reproducibility of experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.