ArticleJournal of immunology (Baltimore, Md. : 1950)2016
Protein Translation Activity: A New Measure of Host Immune Cell Activation.
Article in Journal of immunology (Baltimore, Md. : 1950), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Spatial and functional specialization of human splenic innate lymphoid cells.Nature communications · 2026Article
- Blood-based transcriptomic biomarkers for response to [EJNMMI research · 2025Article
- Cathepsin L-dependent positive selection shapes clonal composition and functional fitness of CD4Nature immunology · 2025Article
- Flow-based In Vivo Method to Enumerate Translating Ribosomes and Translation Elongation Rate.Bio-protocol · 2025Article
- Transcription factor Tox2 is required for metabolic adaptation and tissue residency of ILC3 in the gut.Immunity · 2024Article
- Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate.eLife · 2024Article
- Regulatory T cells suppress CD4+ effector T cell activation by controlling protein synthesis.The Journal of experimental medicine · 2023Article
- Measuring Bulk Translation Activity in Single Mammalian Cells During the Integrated Stress Response.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- A Fas-4-1BB fusion protein converts a death to a pro-survival signal and enhances T cell therapy.The Journal of experimental medicine · 2020Article
- SCENITH: A Flow Cytometry-Based Method to Functionally Profile Energy Metabolism with Single-Cell Resolution.Cell metabolism · 2020Article
- The science of puromycin: From studies of ribosome function to applications in biotechnology.Computational and structural biotechnology journal · 2020Review
- Multi-omics analysis of multiple missions to space reveal a theme of lipid dysregulation in mouse liver.Scientific reports · 2019Article
- Myc controls a distinct transcriptional program in fetal thymic epithelial cells that determines thymus growth.Nature communications · 2019Article
- Parallel analysis of tri-molecular biosynthesis with cell identity and function in single cells.Nature communications · 2019Article
- Mouse adult hematopoietic stem cells actively synthesize ribosomal RNA.RNA (New York, N.Y.) · 2018Article
- Metabolic and Transcriptional Modules Independently Diversify Plasma Cell Lifespan and Function.Cell reports · 2018Article
- The RiboPuromycylation Method (RPM): an Immunofluorescence Technique to Map Translation Sites at the Sub-cellular Level.Bio-protocol · 2018Article
- A Plant Biologist's Toolbox to Study Translation.Frontiers in plant science · 2018Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We describe the in vivo ribopuromycylation (RPM) method, which uses a puromycin-specific Ab to fluorescently label ribosome-bound puromycylated nascent chains, enabling measurement of translational activity via immunohistochemistry or flow cytometry. Tissue staining provides a unique view of virus-induced activation of adaptive, innate, and stromal immune cells. RPM flow precisely quantitates virus-induced activation of lymphocytes and innate immune cells, and it provides a unique measure of immune cell deactivation and quiescence. Using RPM we find that high endothelial cells in draining lymph nodes rapidly increase translation in the first day of vaccinia virus infection. We also find a population of constitutively activated splenic T cells in naive mice and further that most bone marrow T cells activate 3 d after vaccinia virus infection. Bone marrow T cell activation is nonspecific, IL-12-dependent, and induces innate memory T cell phenotypic markers. Thus, RPM measures translational activity to uniquely identify cell populations that participate in the immune response to pathogens, other foreign substances, and autoantigens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.