ArticleThe Plant journal : for cell and molecular biology2016
Dimer/monomer status and in vivo function of salt-bridge mutants of the plant UV-B photoreceptor UVR8.
Article in The Plant journal : for cell and molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- Phosphorylation of the plant photoreceptor UVR8 regulates its dimer/monomer status and enhances UV-B signaling across species.The Plant cell · 2026Article
- Transcription factors interact with Arabidopsis UVR8 photoreceptor at distinct sites to COP1 and RUP proteins.The Plant journal : for cell and molecular biology · 2026Article
- Conservation and divergence of UVR8 photoreceptor-mediated UV-B signaling in Marchantia polymorpha.Plant physiology · 2026Article
- Flavonoid pathway intermediates implicate UVR8 in functions beyond canonical UV-B signaling.Nature communications · 2025Article
- Recent advances in UV-B signalling: interaction of proteins with the UVR8 photoreceptor.Journal of experimental botany · 2025Review
- A seed-specific transcription factor, HSFA9, anticipates UV-B light responses by mimicking the activation of the UV-B receptor in tobacco.The Plant journal : for cell and molecular biology · 2022Article
- Cysteines have a role in conformation of the UVR8 photoreceptor.The Plant journal : for cell and molecular biology · 2022Article
- Low Fluence Ultraviolet-B Promotes Ultraviolet Resistance 8-Modulated Flowering inFrontiers in plant science · 2022Article
- A constitutively monomeric UVR8 photoreceptor confers enhanced UV-B photomorphogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Plant photoreceptors and their signaling components compete for COP1 binding via VP peptide motifs.The EMBO journal · 2019Article
- A FRET method for investigating dimer/monomer status and conformation of the UVR8 photoreceptor.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2019Article
- Native mass spectrometry reveals the conformational diversity of the UVR8 photoreceptor.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- UV RESISTANCE LOCUS 8 FromFrontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
UV RESISTANCE LOCUS8 (UVR8) is a photoreceptor for ultraviolet-B (UV-B) light that initiates photomorphogenic responses in plants. UV-B photoreception causes rapid dissociation of dimeric UVR8 into monomers that interact with CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1) to initiate signal transduction. Experiments with purified UVR8 show that the dimer is maintained by salt-bridge interactions between specific charged amino acids across the dimer interface. However, little is known about the importance of these charged amino acids in determining dimer/monomer status and UVR8 function in plants. Here we evaluate the use of different methods to examine dimer/monomer status of UVR8 and show that mutations of several salt-bridge amino acids affect dimer/monomer status, interaction with COP1 and photoreceptor function of UVR8 in vivo. In particular, the salt-bridges formed between arginine 286 and aspartates 96 and 107 are key to dimer formation. Mutation of arginine 286 to alanine impairs dimer formation, interaction with COP1 and function in vivo, whereas mutation to lysine gives a weakened dimer that is functional in vivo, indicating the importance of the positive charge of the arginine/lysine residue for dimer formation. Notably, a UVR8 mutant in which aspartates 96 and 107 are conservatively mutated to asparagine is strongly impaired in dimer formation but mediates UV-B responses in vivo with a similar dose-response relationship to wild-type. The UV-B responsiveness of this mutant does not correlate with dimer formation and monomerisation, indicating that monomeric UVR8 has the potential for UV-B photoreception, initiating signal transduction and responses in plants.
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