Evidence map›Paper›PMID 27385596›Full record

ArticleExperimental biology and medicine (Maywood, N.J.)2016

Inhibition of microRNA-34a prevents IL-1β-induced extracellular matrix degradation in nucleus pulposus by increasing GDF5 expression.

Wei Liu, Yukun Zhang, Xintong Feng, Shuai Li, Yong Gao, Kun Wang, Yu Song, Shuhua Yang, Ji Tu, Zengwu Shao and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental biology and medicine (Maywood, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
3.9field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 35 citations in OpenAlex.

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  15. The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration.Laboratory investigation; a journal of technical methods and pathology · 2017
    Article
  16. [Research progress of microRNA and its non-viral vector in intervertebral disc degeneration].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2017
    Review
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Wei LiuDepartment of Orthopedics, Tongji Medical College, Huazhong University of Science and Technology, Union Hospital, Wuhan 430022, China.
Yukun ZhangDepartment of Orthopedics, Tongji Medical College, Huazhong University of Science and Technology, Union Hospital, Wuhan 430022, China.
Xintong FengDepartment of Orthopedics, Tongji Medical College, Huazhong University of Science and Technology, Union Hospital, Wuhan 430022, China.
Shuai LiDepartment of Orthopedics, Tongji Medical College, Huazhong University of Science and Technology, Union Hospital, Wuhan 430022, China.
Yong GaoDepartment of Orthopedics, Tongji Medical College, Huazhong University of Science and Technology, Union Hospital, Wuhan 430022, China.
Kun WangDepartment of Orthopedics, Tongji Medical College, Huazhong University of Science and Technology, Union Hospital, Wuhan 430022, China.
Yu SongDepartment of Orthopedics, Tongji Medical College, Huazhong University of Science and Technology, Union Hospital, Wuhan 430022, China.
Shuhua YangDepartment of Orthopedics, Tongji Medical College, Huazhong University of Science and Technology, Union Hospital, Wuhan 430022, China.
Ji TuDepartment of Orthopedics, Tongji Medical College, Huazhong University of Science and Technology, Union Hospital, Wuhan 430022, China.
Zengwu ShaoDepartment of Orthopedics, Tongji Medical College, Huazhong University of Science and Technology, Union Hospital, Wuhan 430022, China.
Cao YangDepartment of Orthopedics, Tongji Medical College, Huazhong University of Science and Technology, Union Hospital, Wuhan 430022, China yangcaom@gmail.com.
Huazhong University of Science and Technology · CNUnion Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accumulating evidence indicates that miRNAs, a class of small non-coding RNAs, are implicated in the pathogenesis of various diseases such as cancer and intervertebral disc degeneration. The aim of this study was to investigate the expression and the biological function of microRNA-34a in intervertebral disc degeneration. In this study, microRNA-34a expression was assessed in nucleus pulposus specimens and in IL-1β-stimulated nucleus pulposus cells by real-time polymerase chain reaction. microRNA-34a functions were investigated by using gain and loss of function experiments in nucleus pulposus cells and a dual luciferase reporter assay in 293T cells. microRNA-34a was dramatically up-regulated in degenerative nucleus pulposus tissues and in IL-1β-stimulated nucleus pulposus cells when compared with controls. Furthermore, growth differentiation factor 5 was identified as a target of microRNA-34a. Aberrant expression of microRNA-34a inhibited growth differentiation factor 5 expression by direct binding to its 3'-untranslated region. This inhibition was abolished by mutation of the microRNA-34a binding sites. In addition, microRNA-34a silencing reversed IL-1β-induced decrease in type II collagen and aggrecan expression in nucleus pulposus cells. This effect was substantially suppressed by growth differentiation factor 5 silencing. Our results suggested that microRNA-34a inhibition prevents IL-1β-induced extracellular matrix degradation in human nucleus pulposus by increasing growth differentiation factor 5 expression. microRNA-34a inhibition may be a novel molecular target for intervertebral disc degeneration treatment through the prevention of nucleus pulposus extracellular matrix degradation.

Indexed as

AdolescentAdultAgedBlotting, WesternExtracellular MatrixFluorescent Antibody TechniqueGrowth Differentiation Factor 5HumansInterleukin-1betaIntervertebral Disc DegenerationMicroRNAsMiddle AgedNucleus PulposusReal-Time Polymerase Chain ReactionScoliosisYoung AdultGDF5 protein, humanGrowth Differentiation Factor 5Interleukin-1betaMicroRNAsMIRN34 microRNA, humanExtracellular matrix degradationgrowth differentiation factor 5IL-1βintervertebral disc degenerationmicroRNA-34anucleus pulposus cell

Identifiers

PMID27385596
PMCPMC5068468
OpenAlexW2467423529

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.