ArticleScientific reports2016
Engineering de novo disulfide bond in bacterial α-type carbonic anhydrase for thermostable carbon sequestration.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 50 citations in OpenAlex.
- Stability and functional consequences of disulfide bond engineering in Aspergillus flavus uricase.Scientific reports · 2025Article
- Protein biochemistry and engineering drive the development of a carbonic anhydrase-based carbon dioxide sequestration strategy.The FEBS journal · 2025Article
- Structural engineering of stabilized, expanded epitope nanoparticle vaccines for HPV.Frontiers in immunology · 2025Article
- Directed evolution of an ultrastable carbonic anhydrase fromComputational and structural biotechnology journal · 2025Article
- Biochemical characterization of a psychrophilic and halotolerant α-carbonic anhydrase from a deep-sea bacterium,AIMS microbiology · 2023Article
- In Silico Analysis of Glucose Oxidase fromBioengineering (Basel, Switzerland) · 2021Article
- A novel hyperthermophilic methylglyoxal synthase: molecular dynamic analysis on the regional fluctuations.Scientific reports · 2021Article
- Enhancement of Pharmaceutical Urate Oxidase Thermostability by Rational Design ofIranian journal of biotechnology · 2020Article
- Overexpression and Purification ofFrontiers in plant science · 2020Article
- Characterization and High-Level Periplasmic Expression of Thermostable α-Carbonic Anhydrase fromInternational journal of molecular sciences · 2019Article
- Crystal Structure of a Highly Thermostable α-Carbonic Anhydrase from Persephonella marina EX-H1.Molecules and cells · 2019Article
- Crystal structure and chemical inhibition of essential schistosome host-interactive virulence factor carbonic anhydrase SmCA.Communications biology · 2019Article
- Enhancing the thermostability ofRSC advances · 2018Article
- FoldX as Protein Engineering Tool: Better Than Random Based Approaches?Computational and structural biotechnology journal · 2018Review
- Microbial Carbonic Anhydrases in Biomimetic Carbon Sequestration for Mitigating Global Warming: Prospects and Perspectives.Frontiers in microbiology · 2017Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exploiting carbonic anhydrase (CA), an enzyme that rapidly catalyzes carbon dioxide hydration, is an attractive biomimetic route for carbon sequestration due to its environmental compatibility and potential economic viability. However, the industrial applications of CA are strongly hampered by the unstable nature of enzymes. In this work, we introduced in silico designed, de novo disulfide bond in a bacterial α-type CA to enhance thermostability. Three variants were selected and expressed in Escherichia coli with an additional disulfide bridge. One of the variants showed great enhancement in terms of both kinetic and thermodynamic stabilities. This improvement could be attributed to the loss of conformational entropy of the unfolded state, showing increased rigidity. The variant showed an upward-shifted optimal temperature and appeared to be thermoactivated, which compensated for the lowered activity at 25 °C. Collectively, the variant constructed by the rapid and effective de novo disulfide engineering can be used as an efficient biocatalyst for carbon sequestration under high temperature conditions.
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