ArticleDevelopment (Cambridge, England)2016
Leapfrogging: primordial germ cell transplantation permits recovery of CRISPR/Cas9-induced mutations in essential genes.
Article in Development (Cambridge, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Generation of chimeric chickens resistant to infection by avian leukemia virus subgroups A, B, D, E, and K through primordial germ cells.Poultry science · 2026Article
- Tissue-Targeted CRISPR-Cas9-Mediated Genome Editing of Multiple Homeologs in FCold Spring Harbor protocols · 2022Article
- Direct allele introgression into pure chicken breeds using Sire Dam Surrogate (SDS) mating.Nature communications · 2021Article
- Early Xenopus gene regulatory programs, chromatin states, and the role of maternal transcription factors.Current topics in developmental biology · 2020Review
- Advancing genetic and genomic technologies deepen the pool for discovery in Xenopus tropicalis.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Review
- Modeling congenital kidney diseases inDisease models & mechanisms · 2019Review
- piRNA-like small RNAs are responsible for the maternal-specific knockdown in the ascidian Ciona intestinalis Type A.Scientific reports · 2018Article
- Tissue-Specific Gene Inactivation inGenetics · 2018Article
- Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus.Journal of visualized experiments : JoVE · 2018Article
- High-efficiency non-mosaic CRISPR-mediated knock-in and indel mutation in F0Development (Cambridge, England) · 2017Article
- Naming CRISPR alleles: endonuclease-mediated mutation nomenclature across species.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- A novel role of the organizer gene Goosecoid as an inhibitor of Wnt/PCP-mediated convergent extension in Xenopus and mouse.Scientific reports · 2017Article
- A frog's view of EphrinB signaling.Genesis (New York, N.Y. : 2000) · 2017Review
- Surrogate broodstock to enhance biotechnology research and applications in aquaculture.Biotechnology advancesReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
CRISPR/Cas9 genome editing is revolutionizing genetic loss-of-function analysis but technical limitations remain that slow progress when creating mutant lines. First, in conventional genetic breeding schemes, mosaic founder animals carrying mutant alleles are outcrossed to produce F1 heterozygotes. Phenotypic analysis occurs in the F2 generation following F1 intercrosses. Thus, mutant analyses will require multi-generational studies. Second, when targeting essential genes, efficient mutagenesis of founders is often lethal, preventing the acquisition of mature animals. Reducing mutagenesis levels may improve founder survival, but results in lower, more variable rates of germline transmission. Therefore, an efficient approach to study lethal mutations would be useful. To overcome these shortfalls, we introduce 'leapfrogging', a method combining efficient CRISPR mutagenesis with transplantation of mutated primordial germ cells into a wild-type host. Tested using Xenopus tropicalis, we show that founders containing transplants transmit mutant alleles with high efficiency. F1 offspring from intercrosses between F0 animals that carry embryonic lethal alleles recapitulate loss-of-function phenotypes, circumventing an entire generation of breeding. We anticipate that leapfrogging will be transferable to other species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.