ArticleScientific reports2016
A native promoter and inclusion of an intron is necessary for efficient expression of GFP or mRFP in Armillaria mellea.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 35 citations in OpenAlex.
- Establishment of an antimetabolite-based transformation system for the wood-decaying basidiomyceteApplied and environmental microbiology · 2025Article
- Establishment of an efficient Agrobacterium tumefaciens-mediated transformation system for an Armillaria species, a host of the fully mycoheterotrophic plant Gastrodia elata.Folia microbiologica · 2025Article
- Protocol for generating protoplasts of Trichoderma koningiopsis and transformation of plasmid DNA.STAR protocols · 2025Article
- Towards engineering agaricomycete fungi for terpenoid production.Journal of industrial microbiology & biotechnology · 2024Review
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- Construction of nucleus-directed fluorescent reporter systems and its application to verification of heterokaryon formation inFrontiers in microbiology · 2022Article
- Efficient expression of heterologous genes by the introduction of the endogenous glyceraldehyde-3-phosphate dehydrogenase gene intron 1 in Ganoderma lucidum.Microbial cell factories · 2021Article
- Enhanced polyethylene glycol (PEG)-mediated protoplast transformation system for the phytopathogenic fungus, Ganoderma boninense.Folia microbiologica · 2021Article
- Epidemiology, Biotic Interactions and Biological Control of Armillarioids in the Northern Hemisphere.Pathogens (Basel, Switzerland) · 2021Review
- The CAG promoter maintains high-level transgene expression in HEK293 cells.FEBS open bio · 2021Article
- Recent developments of tools for genome and metabolome studies in basidiomycete fungi and their application to natural product research.Biology open · 2020Review
- Characterization of the effects of terminators and introns on recombinant gene expression in the basidiomycete Ceriporiopsis subvermispora.Journal of microbiology (Seoul, Korea) · 2020Article
- Fluorescent protein expression in the ectomycorrhizal fungus Laccaria bicolor: a plasmid toolkit for easy use of fluorescent markers in basidiomycetes.Current genetics · 2020Article
- Dual sgRNA-directed gene deletion in basidiomycete Ganoderma lucidum using the CRISPR/Cas9 system.Microbial biotechnology · 2020Article
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- Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins.Microbial cell factories · 2019Article
- Heterologous expression of Zn-binding peptide RaZBP1 from Russula bresadolae does not overcome Zn and Cd detoxification mechanisms in Hebeloma mesophaeum.Folia microbiologica · 2019Article
- Exploring molecular tools for transformation and gene expression in the cultivated edible mushroom Agrocybe aegerita.Molecular genetics and genomics : MGG · 2019Article
- Use of green fluorescent protein to monitor fungal growth in biomass hydrolysate.Biology methods & protocols · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Armillaria mellea is a significant pathogen that causes Armillaria root disease on numerous hosts in forests, gardens and agricultural environments worldwide. Using a yeast-adapted pCAMBIA0380 Agrobacterium vector, we have constructed a series of vectors for transformation of A. mellea, assembled using yeast-based recombination methods. These have been designed to allow easy exchange of promoters and inclusion of introns. The vectors were first tested by transformation into basidiomycete Clitopilus passeckerianus to ascertain vector functionality then used to transform A. mellea. We show that heterologous promoters from the basidiomycetes Agaricus bisporus and Phanerochaete chrysosporium that were used successfully to control the hygromycin resistance cassette were not able to support expression of mRFP or GFP in A. mellea. The endogenous A. mellea gpd promoter delivered efficient expression, and we show that inclusion of an intron was also required for transgene expression. GFP and mRFP expression was stable in mycelia and fluorescence was visible in transgenic fruiting bodies and GFP was detectable in planta. Use of these vectors has been successful in giving expression of the fluorescent proteins GFP and mRFP in A. mellea, providing an additional molecular tool for this pathogen.
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