ArticleInternational journal of molecular sciences2016
Molecular Signals Controlling the Inhibition of Nodulation by Nitrate in Medicago truncatula.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- A comparative meta-proteomic pipeline for the identification of plasmodesmata proteins and regulatory conditions in diverse plant species.BMC biology · 2022Pooled it
- Structure-informed engineering of plant-microbe interactions.The Plant journal : for cell and molecular biology · 2026Review
- Tomato yield enhancement with plasma-activated water as an alternative nitrogen source.BMC plant biology · 2025Article
- Transcriptome analysis reveals nutrient deprivation reduces nitrate content in lettuce (Frontiers in plant science · 2025Article
- The impact of indigenous soil yeasts inoculation on bean (Phaseolus vulgaris) growth.Archives of microbiology · 2022Article
- The Nitrate Transporter MtNPF6.8 Is a Master Sensor of Nitrate Signal in the Primary Root Tip ofFrontiers in plant science · 2022Article
- The Effect of Exogenous Nitrate on LCO Signalling, Cytokinin Accumulation, and Nodule Initiation inGenes · 2021Article
- Co-Inoculation ofPlants (Basel, Switzerland) · 2021Article
- Structural Modeling andFrontiers in plant science · 2021Article
- Genetic Factors Associated With Nodulation and Nitrogen Derived From Atmosphere in a Middle American Common Bean Panel.Frontiers in plant science · 2020Article
- Regulation of Resource Partitioning Coordinates Nitrogen and Rhizobia Responses and Autoregulation of Nodulation in Medicago truncatula.Molecular plant · 2019Article
- Spatio-temporal control of mutualism in legumes helps spread symbiotic nitrogen fixation.eLife · 2017Article
- Molecular Signals in Nodulation Control.International journal of molecular sciences · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence of nitrogen inhibits legume nodule formation, but the mechanism of this inhibition is poorly understood. We found that 2.5 mM nitrate and above significantly inhibited nodule initiation but not root hair curling in Medicago trunatula. We analyzed protein abundance in M. truncatula roots after treatment with either 0 or 2.5 mM nitrate in the presence or absence of its symbiont Sinorhizobium meliloti after 1, 2 and 5 days following inoculation. Two-dimensional gel electrophoresis combined with mass spectrometry was used to identify 106 differentially accumulated proteins responding to nitrate addition, inoculation or time point. While flavonoid-related proteins were less abundant in the presence of nitrate, addition of Nod gene-inducing flavonoids to the Sinorhizobium culture did not rescue nodulation. Accumulation of auxin in response to rhizobia, which is also controlled by flavonoids, still occurred in the presence of nitrate, but did not localize to a nodule initiation site. Several of the changes included defense- and redox-related proteins, and visualization of reactive oxygen species indicated that their induction in root hairs following Sinorhizobium inoculation was inhibited by nitrate. In summary, the presence of nitrate appears to inhibit nodulation via multiple pathways, including changes to flavonoid metabolism, defense responses and redox changes.
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