ArticleIntegrative biology : quantitative biosciences from nano to macro2016
Local extracellular matrix alignment directs cellular protrusion dynamics and migration through Rac1 and FAK.
Article in Integrative biology : quantitative biosciences from nano to macro, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
69 citing papers in PubMed, 115 citations in OpenAlex.
- Preparation and Assembly of the Axial Invasion Chamber for Live-Cell Invadopodia Imaging.Bio-protocol · 2026Article
- Cell-mediated matrix deformations and cell-cell adhesions determine epithelial collective cell migration phenotypes.APL bioengineering · 2025Article
- A structured framework of cytoskeletal proteins and noncentrosomal microtubules promotes the initiation and elongation of invadopodia.Molecular biology of the cell · 2025Article
- Hydrogel Viscoelasticity Modulates Cell Nascent Extracellular Matrix Deposition.Macromolecular rapid communications · 2025Article
- Extracellular matrix architecture promotes immunosuppressive microenvironments in pancreatic cancer.Matrix biology : journal of the International Society for Matrix Biology · 2025Article
- Fiber recruitment drives a phase transition of cell polarization at a critical cell spacing in matrix-mediated tissue remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Revisiting the biophysical aspects of extracellular-matrix-mimicking hydrogels: what cells seeBiomaterials science · 2025Review
- Review
- A Simple Framework for Agent-Based Modeling with Extracellular Matrix.Bulletin of mathematical biology · 2025Article
- Proteolysis and Contractility Regulate Tissue Opening and Wound Healing by Lung Fibroblasts in 3D Microenvironments.Advanced healthcare materials · 2024Article
- Biomimetic Hydrogel Strategies for Cancer Therapy.Gels (Basel, Switzerland) · 2024Review
- Collagen density regulates tip-stalk cell rearrangement during angiogenesis via cellular bioenergetics.APL bioengineering · 2024Article
- Laser speckle rheological microscopy reveals wideband viscoelastic spectra of biological tissues.Science advances · 2024Article
- Endothelial cells metabolically regulate breast cancer invasion toward a microvessel.APL bioengineering · 2023Article
- Tunable Mesoscopic Collagen Island Architectures Modulate Stem Cell Behavior.Advanced materials (Deerfield Beach, Fla.) · 2023Article
- Unravelling cell migration: defining movement from the cell surface.Cell adhesion & migration · 2022Review
- A miniaturized screening platform to identify novel regulators of extracellular matrix alignment.Cancer research communications · 2022Article
- Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment.Journal of visualized experiments : JoVE · 2022Article
- Local extensional flows promote long-range fiber alignment in 3D collagen hydrogels.Biofabrication · 2022Article
- A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment.Journal of visualized experiments : JoVE · 2022Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Cell migration within 3D interstitial microenvironments is sensitive to extracellular matrix (ECM) properties, but the mechanisms that regulate migration guidance by 3D matrix features remain unclear. To examine the mechanisms underlying the cell migration response to aligned ECM, which is prevalent at the tumor-stroma interface, we utilized time-lapse microscopy to compare the behavior of MDA-MB-231 breast adenocarcinoma cells within randomly organized and well-aligned 3D collagen ECM. We developed a novel experimental system in which cellular morphodynamics during initial 3D cell spreading served as a reductionist model for the complex process of matrix-directed 3D cell migration. Using this approach, we found that ECM alignment induced spatial anisotropy of cells' matrix probing by promoting protrusion frequency, persistence, and lengthening along the alignment axis and suppressing protrusion dynamics orthogonal to alignment. Preference for on-axis behaviors was dependent upon FAK and Rac1 signaling and translated across length and time scales such that cells within aligned ECM exhibited accelerated elongation, front-rear polarization, and migration relative to cells in random ECM. Together, these findings indicate that adhesive and protrusive signaling allow cells to respond to coordinated physical cues in the ECM, promoting migration efficiency and cell migration guidance by 3D matrix structure.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.