ArticleHuman genetics2016
CRISPR: a versatile tool for both forward and reverse genetics research.
Article in Human genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Overcoming lentiviral delivery limitations in hard-to-transduce suspension cells for genome-wide CRISPR screening.Molecular therapy. Advances · 2026Article
- Expanding the CRISPR/Cas toolkit: applications in proteomics and theranostics.Frontiers in bioengineering and biotechnology · 2025Review
- Reverse Genetics-Based Methodology for Molecular Virology Study.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Combined CRISPRi and proteomics screening reveal a cohesin-CTCF-bound allele contributing to increased expression of RUVBL1 and prostate cancer progression.American journal of human genetics · 2023Article
- Genome editing technologies, mechanisms and improved production of therapeutic phytochemicals: Opportunities and prospects.Biotechnology and bioengineering · 2023Review
- Characterization of single nucleotide polymorphisms for a forward genetics approach using genetic crosses in C57BL/6 and BALB/c substrains of mice.Experimental animals · 2022Article
- GONAD: A new method for germline genome editing in mice and rats.Development, growth & differentiation · 2021Article
- Article
- Stem Cells and Gene Therapy in Progressive Hearing Loss: the State of the Art.Journal of the Association for Research in Otolaryngology : JARO · 2021Review
- In Search of Molecular Markers for Cerebellar Neurons.International journal of molecular sciences · 2021Review
- A method for determining the cutting efficiency of the CRISPR/Cas system in birch and poplar.Forestry research · 2021Article
- Improving Horticultural Crops via CRISPR/Cas9: Current Successes and Prospects.Plants (Basel, Switzerland) · 2020Review
- Review
- Mapping and editing animal mitochondrial genomes: can we overcome the challenges?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Observation and Control of Gene Expression Noise: Barrier Crossing Analogies Between Drug Resistance and Metastasis.Frontiers in genetics · 2020Article
- CRISPR/Cas9 Genome Editing Technology: A Valuable Tool for Understanding Plant Cell Wall Biosynthesis and Function.Frontiers in plant science · 2020Review
- Disruptive Technology: CRISPR/Cas-Based Tools and Approaches.Molecular diagnosis & therapy · 2019Review
- Generating mouse models for biomedical research: technological advances.Disease models & mechanisms · 2019Review
- Successful production of genome-edited rats by the rGONAD method.BMC biotechnology · 2018Article
- i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases.Genome biology · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Human genetics research employs the two opposing approaches of forward and reverse genetics. While forward genetics identifies and links a mutation to an observed disease etiology, reverse genetics induces mutations in model organisms to study their role in disease. In most cases, causality for mutations identified by forward genetics is confirmed by reverse genetics through the development of genetically engineered animal models and an assessment of whether the model can recapitulate the disease. While many technological advances have helped improve these approaches, some gaps still remain. CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated), which has emerged as a revolutionary genetic engineering tool, holds great promise for closing such gaps. By combining the benefits of forward and reverse genetics, it has dramatically expedited human genetics research. We provide a perspective on the power of CRISPR-based forward and reverse genetics tools in human genetics and discuss its applications using some disease examples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.