Evidence map›Paper›PMID 27384177›Full record

ArticleParasitology research2016

Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody.

Makoto Matsubayashi, Chisa Minoura, Shintaro Kimura, Hiroyuki Tani, Masaru Furuya, Hyun S Lillehoj, Haruo Matsuda, Shigeo Takenaka, Takeshi Hatta, Naotoshi Tsuji and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in Parasitology research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Protection againstInfection and immunity · 2023
    Article
  2. Eimeria proteins: order amidst disorder.Parasites & vectors · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Makoto MatsubayashiDivision of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan.
Chisa MinouraDivision of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan.
Shintaro KimuraDivision of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan.
Hiroyuki TaniDivision of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan.
Masaru FuruyaDivision of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan.
Hyun S LillehojAnimal Biosciences and Biotechnology Laboratory, Agricultural Research Services, United States Department of Agriculture, Beltsville, MD, 20705, USA.
Haruo MatsudaLaboratory of Immunobiology, Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi, Hiroshima, 739-8528, Japan.
Shigeo TakenakaDivision of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan.
Takeshi HattaDepartment of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa, 252-0374, Japan.
Naotoshi TsujiDepartment of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa, 252-0374, Japan.
Kazumi SasaiDivision of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan. ksasai@vet.osakafu-u.ac.jp.
Osaka Prefecture University · JPKitasato University · JPHiroshima University · JPUnited States Department of Agriculture · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the poultry industry, Eimeria spp. is one of the important pathogens which cause significant economic losses. We have previously generated a chicken monoclonal antibody (mAb), 6D-12-G10, with specificity for an antigen located in the apical cytoskeleton of Eimeria acervulina and with cross-reactive among Apicomplexan parasites, including other Eimeria spp., Toxoplasma, Neospora, and Cryptosporidium spp. Furthermore, the protein of Cryptosporidium parvum recognized by the 6D-12-G10 has been identified as elongation factor-1α (EF-1α). In the present study, to identify the target molecule of E. acervulina by the mAb, we performed two-dimensional Western blotting analysis. Finally, we found two positive molecules which are identified as EF-1α and a related protein. Our previous finding using C. parvum and the results in this study suggest that EF-1α could be associated with the invasion facilitated by the cytoskeleton at the apical region of zoites.

Indexed as

Amino Acid SequenceAnimalsAntibodies, MonoclonalAntibodies, ProtozoanAntigens, ProtozoanBlotting, WesternChickensCoccidiosisCross ReactionsCryptosporidium parvumEimeriaEnzyme-Linked Immunosorbent AssayNeosporaPeptide Elongation Factor 1Poultry DiseasesSporozoitesAntibodies, MonoclonalAntibodies, ProtozoanAntigens, ProtozoanPeptide Elongation Factor 1Chicken monoclonal antibodyConoidEimeria acervulinaElongation factor-1α

Identifiers

PMID27384177
OpenAlexW2471098953

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.