ArticleNature2016
Resolving early mesoderm diversification through single-cell expression profiling.
Article in Nature, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 168 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
168 citing papers in PubMed, 316 citations in OpenAlex.
- AXIN1 and AXIN2 regulate the WNT-signaling landscape to promote distinct mesoderm programs.Developmental cell · 2026Article
- Shortcomings of silhouette in single-cell integration benchmarking.Nature biotechnology · 2026Article
- Article
- Identifying batch-integrated domains from spatial transcriptomics via graph autoencoder with contrastive learning based on cross-modality and data augmentation.Briefings in bioinformatics · 2026Article
- Review
- Integrating polygenic signals and single-cell multiomics identifies cell-type-specific regulomes critical for immune- and aging-related diseases.Nature aging · 2026Article
- Integrated Single-cell Analysis Uncovers Regulatory Logic of Cranial Ectoderm Development.bioRxiv : the preprint server for biology · 2025Article
- Fzd2 orchestrates canonical and non-canonical Wnt signaling to regulate lung development and fibrosis.Cell communication and signaling : CCS · 2025Article
- Competing dynamic gene regulatory networks involved in fibroblast reprogramming to hematopoietic progenitor cells.Stem cell reports · 2025Article
- Single-cell multi-omics delineates the dynamics of distinct epigenetic codes coordinating mouse gastrulation.BMC genomics · 2025Article
- Single-cell omics: experimental workflow, data analyses and applications.Science China. Life sciences · 2025Review
- Stem cell culture conditions affect in vitro differentiation potential and mouse gastruloid formation.PloS one · 2025Article
- Progress and potential of brain organoids in epilepsy research.Stem cell research & therapy · 2024Review
- An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration.Developmental cell · 2024Article
- BANMF-S: a blockwise accelerated non-negative matrix factorization framework with structural network constraints for single cell imputation.Briefings in bioinformatics · 2024Article
- Leveraging neighborhood representations of single-cell data to achieve sensitive DE testing with miloDE.Genome biology · 2024Article
- Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation.Journal of visualized experiments : JoVE · 2024Article
- Tracking early mammalian organogenesis - prediction and validation of differentiation trajectories at whole organism scale.Development (Cambridge, England) · 2024Article
- Cardiac Development at a Single-Cell Resolution.Advances in experimental medicine and biology · 2024Article
- Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation.Nature communications · 2023Article
108 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 1 country.
Funding
Abstract
In mammals, specification of the three major germ layers occurs during gastrulation, when cells ingressing through the primitive streak differentiate into the precursor cells of major organ systems. However, the molecular mechanisms underlying this process remain unclear, as numbers of gastrulating cells are very limited. In the mouse embryo at embryonic day 6.5, cells located at the junction between the extra-embryonic region and the epiblast on the posterior side of the embryo undergo an epithelial-to-mesenchymal transition and ingress through the primitive streak. Subsequently, cells migrate, either surrounding the prospective ectoderm contributing to the embryo proper, or into the extra-embryonic region to form the yolk sac, umbilical cord and placenta. Fate mapping has shown that mature tissues such as blood and heart originate from specific regions of the pre-gastrula epiblast, but the plasticity of cells within the embryo and the function of key cell-type-specific transcription factors remain unclear. Here we analyse 1,205 cells from the epiblast and nascent Flk1(+) mesoderm of gastrulating mouse embryos using single-cell RNA sequencing, representing the first transcriptome-wide in vivo view of early mesoderm formation during mammalian gastrulation. Additionally, using knockout mice, we study the function of Tal1, a key haematopoietic transcription factor, and demonstrate, contrary to previous studies performed using retrospective assays, that Tal1 knockout does not immediately bias precursor cells towards a cardiac fate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.