ArticleACS nano2016
Nanoanalytical Electron Microscopy Reveals a Sequential Mineralization Process Involving Carbonate-Containing Amorphous Precursors.
Article in ACS nano, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 72 citations in OpenAlex.
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- Mechanical Overloading-Induced Nanomineral Crystal Perturbation from the Osteochondral Interface: A Potential Initiator of Osteoarthritis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Novel Ti6Al4V Surface Treatment for Subperiosteal Dental Implants (Part II): Matrix Deposition and Osteogenic Markers.Materials (Basel, Switzerland) · 2026Article
- Physical and chemical niche of human growth plate for polarized bone development.Nature communications · 2025Article
- A tough soft-hard interface in the human knee joint driven by multiscale toughening mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Decoding the mechanical characteristics of the human anterior cruciate ligament entheses through graduated mineralization interfaces.Nature communications · 2024Article
- The mechanism of biomineralization: Progress in mineralization from intracellular generation to extracellular deposition.The Japanese dental science review · 2023Review
- Research progress of biomimetic materials in oral medicine.Journal of biological engineering · 2023Review
- Triggered metabolism of adenosine triphosphate as an explanation for the chemical heterogeneity of heterotopic ossification.Communications chemistry · 2023Article
- Inorganic phosphate in growing calcium carbonate abalone shell suggests a shared mineral ancestral precursor.Nature communications · 2022Article
- Ion Pathways in Biomineralization: Perspectives on Uptake, Transport, and Deposition of Calcium, Carbonate, and Phosphate.Journal of the American Chemical Society · 2021Article
- Janus 3D printed dynamic scaffolds for nanovibration-driven bone regeneration.Nature communications · 2021Article
- Micro-Nano Surface Characterization and Bioactivity of a Calcium Phosphate-Incorporated Titanium Implant Surface.Journal of functional biomaterials · 2021Article
- Mitochondrial Dysfunction: Cause or Consequence of Vascular Calcification?Frontiers in cell and developmental biology · 2021Review
- Combined Effect of Midazolam and Bone Morphogenetic Protein-2 for Differentiation Induction from C2C12 Myoblast Cells to Osteoblasts.Pharmaceutics · 2020Article
- Silicon nitride enhances osteoprogenitor cell growth and differentiation via increased surface energy and formation of amide and nanocrystalline HA for craniofacial reconstruction.Medical devices & sensors · 2019Article
- Article
- Transformation of amorphous calcium phosphate to bone-like apatite.Nature communications · 2018Article
- Phosphorus Balance in Adolescent Girls and the Effect of Supplemental Dietary Calcium.JBMR plus · 2018Article
- Transformation of ACC into aragonite and the origin of the nanogranular structure of nacre.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
A direct observation and an in-depth characterization of the steps by which bone mineral nucleates and grows in the extracellular matrix during the earliest stages of maturation, using relevant biomineralization models as they grow into mature bone mineral, is an important research goal. To better understand the process of bone mineralization in the extracellular matrix, we used nanoanalytical electron microscopy techniques to examine an in vitro model of bone formation. This study demonstrates the presence of three dominant CaP structures in the mineralizing osteoblast cultures: <80 nm dense granules with a low calcium to phosphate ratio (Ca/P) and crystalline domains; calcium phosphate needles emanating from a focus: "needle-like globules" (100-300 nm in diameter) and mature mineral, both with statistically higher Ca/P compared to that of the dense granules. Many of the submicron granules and globules were interspersed around fibrillar structures containing nitrogen, which are most likely the signature of the organic phase. With high spatial resolution electron energy loss spectroscopy (EELS) mapping, spatially resolved maps were acquired showing the distribution of carbonate within each mineral structure. The carbonate was located in the middle of the granules, which suggested the nucleation of the younger mineral starts with a carbonate-containing precursor and that this precursor may act as seed for growth into larger, submicron-sized, needle-like globules of hydroxyapatite with a different stoichiometry. Application of analytical electron microscopy has important implications in deciphering both how normal bone forms and in understanding pathological mineralization.
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