ArticlePloS one2016
Crystal Structure of Bacillus subtilis Cysteine Desulfurase SufS and Its Dynamic Interaction with Frataxin and Scaffold Protein SufU.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- The structural and functional analysis of mycobacteria cysteine desulfurase-loaded encapsulin.Communications biology · 2024Article
- Structural and Biochemical Characterization ofACS omega · 2022Article
- Structural diversity of cysteine desulfurases involved in iron-sulfur cluster biosynthesis.Biophysics and physicobiology · 2022Article
- The sulfur formation system mediating extracellular cysteine-cystine recycling in Fervidobacterium islandicum AW-1 is associated with keratin degradation.Microbial biotechnology · 2021Article
- Occurrence, Evolution and Specificities of Iron-Sulfur Proteins and Maturation Factors in Chloroplasts from Algae.International journal of molecular sciences · 2021Review
- Exploring the selenium-over-sulfur substrate specificity and kinetics of a bacterial selenocysteine lyase.Biochimie · 2021Article
- Fe-S cluster biogenesis by the bacterial Suf pathway.Biochimica et biophysica acta. Molecular cell research · 2020Review
- Structural evidence for a latch mechanism regulating access to the active site of SufS-family cysteine desulfurases.Acta crystallographica. Section D, Structural biology · 2020Article
- Maturation of the respiratory complex II flavoprotein.Current opinion in structural biology · 2019Review
- Direct observation of intermediates in the SufS cysteine desulfurase reaction reveals functional roles of conserved active-site residues.The Journal of biological chemistry · 2019Article
- Iron-sulfur clusters biogenesis by the SUF machinery: close to the molecular mechanism understanding.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Review
- Structure and functional dynamics of the mitochondrial Fe/S cluster synthesis complex.Nature communications · 2017Article
- Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the ArchaeonArchaea (Vancouver, B.C.) · 2017Article
- The B1 Protein Guides the Biosynthesis of a Lasso Peptide.Scientific reports · 2016Article
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Authors and funding
7 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The biosynthesis of iron sulfur (Fe-S) clusters in Bacillus subtilis is mediated by a SUF-type gene cluster, consisting of the cysteine desulfurase SufS, the scaffold protein SufU, and the putative chaperone complex SufB/SufC/SufD. Here, we present the high-resolution crystal structure of the SufS homodimer in its product-bound state (i.e., in complex with pyrodoxal-5'-phosphate, alanine, Cys361-persulfide). By performing hydrogen/deuterium exchange (H/DX) experiments, we characterized the interaction of SufS with SufU and demonstrate that SufU induces an opening of the active site pocket of SufS. Recent data indicate that frataxin could be involved in Fe-S cluster biosynthesis by facilitating iron incorporation. H/DX experiments show that frataxin indeed interacts with the SufS/SufU complex at the active site. Our findings deepen the current understanding of Fe-S cluster biosynthesis, a complex yet essential process, in the model organism B. subtilis.
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