ArticlePlant physiology2016
Suppressing Farnesyl Diphosphate Synthase Alters Chloroplast Development and Triggers Sterol-Dependent Induction of Jasmonate- and Fe-Related Responses.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Farnesol and geranylgeraniol in plant reproduction: insights from Arabidopsis and beyond.Journal of experimental botany · 2026Review
- Rab geranylgeranyl transferase activity is required for proper sterol biosynthesis in Arabidopsis thaliana.Plant & cell physiology · 2026Article
- Effect of salicylic acid on morphological traits and the expression of key rubber biosynthesis genes in Parthenium argentatum A. Gray.PloS one · 2026Article
- Analysis of Stress Response Genes in Microtuberization of PotatoPlants (Basel, Switzerland) · 2024Article
- Non-Targeted Metabolomic Analysis ofMetabolites · 2023Article
- Genome-Wide Association Study of Seed Morphology Traits in Senegalese Sorghum Cultivars.Plants (Basel, Switzerland) · 2023Article
- How plants synthesize coenzyme Q.Plant communications · 2022Review
- Molecular Cloning and Functional Analysis ofFoods (Basel, Switzerland) · 2022Article
- Activation of the plant mevalonate pathway by extracellular ATP.Nature communications · 2022Article
- Effects of impaired steryl ester biosynthesis on tomato growth and developmental processes.Frontiers in plant science · 2022Article
- Ornithine decarboxylase genes contribute to S-RNase-independent pollen rejection.Plant physiology · 2021Article
- The Role of Membranes and Lipid-Protein Interactions in the Mg-Branch of Tetrapyrrole Biosynthesis.Frontiers in plant science · 2021Review
- Functional Gene Network of Prenyltransferases inMolecules (Basel, Switzerland) · 2019Review
- Inactivation of UDP-Glucose Sterol Glucosyltransferases EnhancesFrontiers in plant science · 2019Article
- Transcriptome Analysis Reveals Candidate Genes Associated with Leaf Etiolation of a Cytoplasmic Male Sterility Line in Chinese Cabbage (Brassica Rapa L. ssp. Pekinensis).International journal of molecular sciences · 2018Article
- Improved fruit α-tocopherol, carotenoid, squalene and phytosterol contents through manipulation of Brassica juncea 3-HYDROXY-3-METHYLGLUTARYL-COA SYNTHASE1 in transgenic tomato.Plant biotechnology journal · 2018Article
- Complex interplays between phytosterols and plastid development.Plant signaling & behavior · 2017Article
- Tomato UDP-Glucose Sterol Glycosyltransferases: A Family of Developmental and Stress Regulated Genes that Encode Cytosolic and Membrane-Associated Forms of the Enzyme.Frontiers in plant science · 2017Article
- Differential impact of impaired steryl ester biosynthesis on the metabolome of tomato fruits and seeds.Physiologia plantarumArticle
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Farnesyl diphosphate synthase (FPS) catalyzes the synthesis of farnesyl diphosphate from isopentenyl diphosphate and dimethylallyl diphosphate. Arabidopsis (Arabidopsis thaliana) contains two genes (FPS1 and FPS2) encoding FPS. Single fps1 and fps2 knockout mutants are phenotypically indistinguishable from wild-type plants, while fps1/fps2 double mutants are embryo lethal. To assess the effect of FPS down-regulation at postembryonic developmental stages, we generated Arabidopsis conditional knockdown mutants expressing artificial microRNAs devised to simultaneously silence both FPS genes. Induction of silencing from germination rapidly caused chlorosis and a strong developmental phenotype that led to seedling lethality. However, silencing of FPS after seed germination resulted in a slight developmental delay only, although leaves and cotyledons continued to show chlorosis and altered chloroplasts. Metabolomic analyses also revealed drastic changes in the profile of sterols, ubiquinones, and plastidial isoprenoids. RNA sequencing and reverse transcription-quantitative polymerase chain reaction transcriptomic analysis showed that a reduction in FPS activity levels triggers the misregulation of genes involved in biotic and abiotic stress responses, the most prominent one being the rapid induction of a set of genes related to the jasmonic acid pathway. Down-regulation of FPS also triggered an iron-deficiency transcriptional response that is consistent with the iron-deficient phenotype observed in FPS-silenced plants. The specific inhibition of the sterol biosynthesis pathway by chemical and genetic blockage mimicked these transcriptional responses, indicating that sterol depletion is the primary cause of the observed alterations. Our results highlight the importance of sterol homeostasis for normal chloroplast development and function and reveal important clues about how isoprenoid and sterol metabolism is integrated within plant physiology and development.
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