ArticleJournal of experimental botany2016
Two tomato GDP-D-mannose epimerase isoforms involved in ascorbate biosynthesis play specific roles in cell wall biosynthesis and development.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 71 citations in OpenAlex.
- Integrated physiological, biochemical and transcriptomic analyses revealed key genes regulating ascorbic acid biosynthesis during sweet potato development.Food chemistry. Molecular sciences · 2025Article
- Storming the barricades of rhamnogalacturonan-II synthesis and function.The Plant cell · 2025Review
- Solanaceae pan-genomes reveal extensive fractionation and functional innovation of duplicated genes.Plant communications · 2025Article
- Establishment and Validation of an EfficientInternational journal of molecular sciences · 2024Article
- Characterization of the GGP gene family in potato (Solanum tuberosum L.) and Pepper (Capsicum annuum L.) and its expression analysis under hormonal and abiotic stresses.Scientific reports · 2024Article
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- A mutation in CsGME encoding GDP-mannose 3,5-epimerase results in little and wrinkled leaf in cucumber.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Genetics aspect of vitamin C (Ascorbic Acid) biosynthesis and signaling pathways in fruits and vegetables crops.Functional & integrative genomics · 2024Review
- Effect of light on ascorbic acid biosynthesis and bioinformatics analysis of related genes in Chinese chives.PloS one · 2024Article
- Comparative Metabolomic and Transcriptomic Analyses Reveal Distinct Ascorbic Acid (AsA) Accumulation Patterns between PCA and PCNA Persimmon Developing Fruit.International journal of molecular sciences · 2023Article
- Multi-omics quantitative data of tomato fruit unveils regulation modes of least variable metabolites.BMC plant biology · 2023Article
- Overexpressing Vitamin C Defective 2 reduces fertility and alters Ca2+ signals in Arabidopsis pollen.Plant physiology · 2023Article
- Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway.Frontiers in plant science · 2023Review
- Molecular cloning and functional analysis of a Chrysanthemum vestitum GME homolog that enhances drought tolerance in transgenic tobacco.Scientific reports · 2022Article
- Metabolism and Regulation of Ascorbic Acid in Fruits.Plants (Basel, Switzerland) · 2022Review
- Colonization with non-mycorrhizal culturable endophytic fungi enhances orchid growth and indole acetic acid production.BMC microbiology · 2022Article
- Comparative QTL mapping for male sterility of cultivated strawberry (Breeding science · 2021Article
- Label-free quantitative proteomics analysis of jujube (RSC advances · 2021Article
- GDP-Mannose 3,5-Epimerase: A View on Structure, Mechanism, and Industrial Potential.Frontiers in molecular biosciences · 2021Review
- A CCAAT-binding factor, SlNFYA10, negatively regulates ascorbate accumulation by modulating the D-mannose/L-galactose pathway in tomato.Horticulture research · 2020Article
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16 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
GDP-D-mannose epimerase (GME, EC 5.1.3.18) converts GDP-D-mannose to GDP-L-galactose, and is considered to be a central enzyme connecting the major ascorbate biosynthesis pathway to primary cell wall metabolism in higher plants. Our previous work demonstrated that GME is crucial for both ascorbate and cell wall biosynthesis in tomato. The aim of the present study was to investigate the respective role in ascorbate and cell wall biosynthesis of the two SlGME genes present in tomato by targeting each of them through an RNAi-silencing approach. Taken individually SlGME1 and SlGME2 allowed normal ascorbate accumulation in the leaf and fruits, thus suggesting the same function regarding ascorbate. However, SlGME1 and SlGME2 were shown to play distinct roles in cell wall biosynthesis, depending on the tissue considered. The RNAi-SlGME1 plants harbored small and poorly seeded fruits resulting from alterations of pollen development and of pollination process. In contrast, the RNAi-SlGME2 plants exhibited vegetative growth delay while fruits remained unaffected. Analysis of SlGME1- and SlGME2-silenced seeds and seedlings further showed that the dimerization state of pectin rhamnogalacturonan-II (RG-II) was altered only in the RNAi-SlGME2 lines. Taken together with the preferential expression of each SlGME gene in different tomato tissues, these results suggest sub-functionalization of SlGME1 and SlGME2 and their specialization for cell wall biosynthesis in specific tomato tissues.
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