ArticleNucleic acids research2016
Binding properties of YjeQ (RsgA), RbfA, RimM and Era to assembly intermediates of the 30S subunit.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Elucidating structure-function relationships in the mammalian nucleolus.Nature reviews. Molecular cell biology · 2026Review
- Mechanistic insights into E. coli recovery from growth arrest.Nature communications · 2026Article
- Mechanistic insights intobioRxiv : the preprint server for biology · 2026Article
- KsgA facilitates ribosomal small subunit maturation by proofreading a key structural lesion.Nature structural & molecular biology · 2023Article
- Comprehensive essentiality analysis of themBio · 2023Article
- White and green striate leaves 1, predicted to encode a 16S rRNA processing protein, plays a critical role in the processing of chloroplast ribosomes in maize (Molecular breeding : new strategies in plant improvement · 2023Article
- Uncovering the Important Genetic Factors for Growth during Cefotaxime-Gentamicin Combination Treatment inAntibiotics (Basel, Switzerland) · 2023Article
- Emerging Quantitative Biochemical, Structural, and Biophysical Methods for Studying Ribosome and Protein-RNA Complex Assembly.Biomolecules · 2023Review
- Purification and Characterization of Authentic 30S Ribosomal Precursors Induced by Heat Shock.International journal of molecular sciences · 2023Article
- GTPase Era at the heart of ribosome assembly.Frontiers in molecular biosciences · 2023Review
- Structural Basis for the C-Terminal Domain ofBiomolecules · 2021Article
- Alternative conformations and motions adopted by 30S ribosomal subunits visualized by cryo-electron microscopy.RNA (New York, N.Y.) · 2020Article
- Functional Analysis of BipA in E. coli Reveals the Natural Plasticity of 50S Subunit Assembly.Journal of molecular biology · 2020Article
- RbfA and IF3 couple ribosome biogenesis and translation initiation to increase stress tolerance.Nucleic acids research · 2020Article
- How the initiating ribosome copes with ppGpp to translate mRNAs.PLoS biology · 2020Article
- The Impact of the Stringent Response on TRAFAC GTPases and Prokaryotic Ribosome Assembly.Cells · 2019Review
- Role of Era in assembly and homeostasis of the ribosomal small subunit.Nucleic acids research · 2019Article
- Role of ribosome assembly in Escherichia coli ribosomal RNA degradation.Nucleic acids research · 2018Article
- Reconstitution of 30S ribosomal subunits in vitro using ribosome biogenesis factors.RNA (New York, N.Y.) · 2018Article
- A metastable rRNA junction essential for bacterial 30S biogenesis.Nucleic acids research · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Our understanding regarding the function of YjeQ (also called RsgA), RbfA, RimM and Era in ribosome biogenesis has been derived in part from the study of immature 30S particles that accumulate in null strains lacking one of these factors. However, their mechanistic details are still unknown. Here, we demonstrate that these immature particles are not dead-end products of assembly, but progress into mature 30S subunits. Mass spectrometry analysis revealed that in vivo the occupancy level of these factors in these immature 30S particles is below 10% and that the concentration of factors does not increase when immature particles accumulate in cells. We measured by microscale thermophoresis that YjeQ and Era binds to the mature 30S subunit with high affinity. However, the binding affinity of these factors to the immature particles and of RimM and RbfA to mature or immature particles was weak, suggesting that binding is not occurring at physiological concentrations. These results suggest that in the absence of these factors, the immature particles evolve into a thermodynamically stable intermediate that exhibits low affinity for the assembly factors. These results imply that the true substrates of YjeQ, RbfA, RimM and Era are immature particles that precede the ribosomal particles accumulating in the knockouts strains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.