ArticlePlant biotechnology journal2017
Genetic enhancement of palmitic acid accumulation in cotton seed oil through RNAi down-regulation of ghKAS2 encoding β-ketoacyl-ACP synthase II (KASII).
Article in Plant biotechnology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 57 citations in OpenAlex.
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- GWAS-based identification of GmKASII and its interaction with GmFATA1B reveals their role in soybean oil composition and accumulation.The Plant journal : for cell and molecular biology · 2026Article
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- Seed-specific expression of AtWRI1 enhanced the yield of cotton seed oil.Scientific reports · 2024Article
- Regulation of Oil Biosynthesis and Genetic Improvement in Plants: Advances and Prospects.Genes · 2024Review
- Oil candidate genes in seeds of cotton (Gossypium hirsutum L.) and functional validation of GhPXN1.Biotechnology for biofuels and bioproducts · 2023Article
- A comprehensive overview of cotton genomics, biotechnology and molecular biological studies.Science China. Life sciences · 2023Review
- Metabolomics and Transcriptomics Analyses Reveals the Molecular Regulatory Mechanisms of Walnut (International journal of molecular sciences · 2023Article
- Metabolomics and Transcriptomics Provide Insights into Lipid Biosynthesis in the Embryos of Walnut (Plants (Basel, Switzerland) · 2023Article
- Development of the engineered "glanded plant and glandless seed" cotton.Food chemistry. Molecular sciences · 2022Article
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- Identification and analysis of oil candidate genes reveals the molecular basis of cottonseed oil accumulation in Gossypium hirsutum L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Development of Intron Polymorphism Markers and Their Association With Fatty Acid Component Variation in Oil Palm.Frontiers in plant science · 2022Article
- Genetics, Breeding and Genetic Engineering to Improve Cottonseed Oil and Protein: A Review.Frontiers in plant science · 2022Review
- A global survey of the gene network and key genes for oil accumulation in cultivated tetraploid cottons.Plant biotechnology journal · 2021Article
- Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton.BMC genomics · 2020Article
- De novo transcriptome assembly for the five major organs of Zanthoxylum armatum and the identification of genes involved in terpenoid compound and fatty acid metabolism.BMC genomics · 2020Article
- A Synergistic Genetic Engineering Strategy Induced Triacylglycerol Accumulation in Potato (Frontiers in plant science · 2020Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Palmitic acid (C16:0) already makes up approximately 25% of the total fatty acids in the conventional cotton seed oil. However, further enhancements in palmitic acid content at the expense of the predominant unsaturated fatty acids would provide increased oxidative stability of cotton seed oil and also impart the high melting point required for making margarine, shortening and confectionary products free of trans fatty acids. Seed-specific RNAi-mediated down-regulation of β-ketoacyl-ACP synthase II (KASII) catalysing the elongation of palmitoyl-ACP to stearoyl-ACP has succeeded in dramatically increasing the C16 fatty acid content of cotton seed oil to well beyond its natural limits, reaching up to 65% of total fatty acids. The elevated C16 levels were comprised of predominantly palmitic acid (C16:0, 51%) and to a lesser extent palmitoleic acid (C16:1, 11%) and hexadecadienoic acid (C16:2, 3%), and were stably inherited. Despite of the dramatic alteration of fatty acid composition and a slight yet significant reduction in oil content in these high-palmitic (HP) lines, seed germination remained unaffected. Regiochemical analysis of triacylglycerols (TAG) showed that the increased levels of palmitic acid mainly occurred at the outer positions, while C16:1 and C16:2 were predominantly found in the sn-2 position in both TAG and phosphatidylcholine. Crossing the HP line with previously created high-oleic (HO) and high-stearic (HS) genotypes demonstrated that HP and HO traits could be achieved simultaneously; however, elevation of stearic acid was hindered in the presence of high level of palmitic acid.
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