ArticleScientific reports2016
Defined enzyme cocktail from the anaerobic fungus Orpinomyces sp. strain C1A effectively releases sugars from pretreated corn stover and switchgrass.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 58 citations in OpenAlex.
- Mining expansin-like proteins from rumen microbiota and functional characterization of two anaerobic fungal expansin-like proteins.Journal of animal science and biotechnology · 2025Article
- Anaerobic fungi: effective warriors in lignocellulosic biomass degradation and fermentation.FEMS microbiology ecology · 2025Review
- Article
- Patterns and determinants of the global herbivorous mycobiome.Nature communications · 2023Article
- Dynamic changes in fecal microbiota in donkey foals during weaning: From pre-weaning to post-weaning.Frontiers in microbiology · 2023Article
- Review
- Anaerobic Fungal Mevalonate Pathway Genomic Biases Lead to Heterologous Toxicity Underpredicted by Codon Adaptation Indices.Microorganisms · 2021Article
- Isolation and Biochemical Characterization of Six Anaerobic Fungal Strains from Zoo Animal Feces.Microorganisms · 2021Article
- The Anaerobic Fungi: Challenges and Opportunities for Industrial Lignocellulosic Biofuel Production.Microorganisms · 2021Review
- Characterization and Phylogenetic Analysis of a Novel GH43 β-Xylosidase FromFrontiers in fungal biology · 2021Article
- Innovations in CAZyme gene diversity and its modification for biorefinery applications.Biotechnology reports (Amsterdam, Netherlands) · 2020Review
- Genomic and proteomic biases inform metabolic engineering strategies for anaerobic fungi.Metabolic engineering communications · 2020Article
- Nucleotide and protein researches on anaerobic fungi during four decades.Journal of animal science and technology · 2020Review
- Expression Profiling of Plant Cell Wall-Degrading Enzyme Genes inFrontiers in physiology · 2020Article
- Enzymes of early-diverging, zoosporic fungi.Applied microbiology and biotechnology · 2019Review
- The biotechnological potential of anaerobic fungi on fiber degradation and methane production.World journal of microbiology & biotechnology · 2018Review
- Development of an RNA interference (RNAi) gene knockdown protocol in the anaerobic gut fungusPeerJ · 2018Article
- Hydrolytic potential of five fungal supernatants to enhance a commercial enzyme cocktail.Biotechnology letters · 2017Article
- Production of a recombinant swollenin from Trichoderma harzianum in Escherichia coli and its potential synergistic role in biomass degradation.Microbial cell factories · 2017Article
- Key Ecological Roles for Zoosporic True Fungi in Aquatic Habitats.Microbiology spectrum · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The anaerobic fungus Orpinomyces strain C1A is capable of growth on various types of lignocellulosic substrates, and harbors an impressive reservoir of carbohydrate active enzymes (CAZymes). Using a minimum enzyme cocktail strategy, we constituted a four-component lignocellulolytic cocktail derived from highly transcribed C1A, and evaluated its efficacy against pretreated corn stover and switchgrass. Hydrolysis yields ranged between 65-77.4%, depending on the lignocellulosic substrate and pretreatment applied. Addition of a highly expressed anaerobic fungal swollenin improved hydrolysis yields by up to 7%. Compared to the commercial cocktail CTec2, these anaerobic fungal cocktails provided comparable or slightly lower hydrolysis yields. Further, the differences in efficacy between commercial and anaerobic cocktails were often only realized after extended (168 hr) incubations. Under certain conditions, the hydrolysis yields of the anaerobic fungal cocktail was slightly superior to that realized by CTec2. We attribute the observed high hydrolysis yields to the high specific activity and affinity of the individual enzymes of the cocktail, as well as the high level of synergy and multi-functionality observed in multiple components. Collectively, this effort provides a novel platform for constructing highly effective enzymes for biofuel production and represents the first lignocellulolytic enzyme cocktail created from anaerobic fungal enzymes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.