ArticleScientific reports2016
"Calibration-on-the-spot": How to calibrate an EMCCD camera from its images.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
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- Impact of Synergy Partner Cel7B on Cel7A Binding Rates: Insights from Single-Molecule Data.The journal of physical chemistry. B · 2024Article
- A gain series method for accurate EMCCD calibration.Scientific reports · 2021Article
- Cega: a single particle segmentation algorithm to identify moving particles in a noisy system.Molecular biology of the cell · 2021Article
- Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules.Nature communications · 2021Article
- Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells.Nature communications · 2019Article
- Precise Time Superresolution by Event Correlation Microscopy.Biophysical journal · 2019Article
- Fidelity test for through-focus or volumetric type of optical imaging methods.Optics express · 2018Article
- Through-focus or volumetric type of optical imaging methods: a review.Journal of biomedical optics · 2018Review
- Enhancing optical microscopy illumination to enable quantitative imaging.Scientific reports · 2018Article
- Subnanometer localization accuracy in widefield optical microscopy.Light, science & applications · 2018Article
- An efficient method for measuring the internal parameters of optical cameras based on optical fibres.Scientific reports · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to count photons with a camera, the camera must be calibrated. Photon counting is necessary, e.g., to determine the precision of localization-based super-resolution microscopy. Here we present a protocol that calibrates an EMCCD camera from information contained in isolated, diffraction-limited spots in any image taken by the camera, thus making dedicated calibration procedures redundant by enabling calibration post festum, from images filed without calibration information.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.