ArticlePLoS genetics2016
Next-Generation "-omics" Approaches Reveal a Massive Alteration of Host RNA Metabolism during Bacteriophage Infection of Pseudomonas aeruginosa.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
75 citing papers in PubMed, 121 citations in OpenAlex.
- Bacteriophage PAK_P3 genome structuration and dynamics during infection ofScience advances · 2026Article
- Abundance-activity decoupling in sulfur-cycling bacteria reflects viral infection types in meromictic lakes.The ISME journal · 2026Article
- Multiomics Approaches for Bacteriophage-Based Biocontrol Applications: A Review of Metabolomics, Transcriptomics, and Proteomics.BioMed research international · 2026Review
- Viral infection collapses intracytoplasmic membrane integrity and autotrophic metabolism in ammonia-oxidizingISME communications · 2026Article
- Viruses and virus satellites of haloarchaea and their nanosized DPANN symbionts reveal intricate nested interactions.Nature microbiology · 2025Article
- Phages with a broad host range are common across ecosystems.Nature microbiology · 2025Article
- Metabolic remodeling of microorganisms by mobile genetic elements alters mutualistic community composition.mSystems · 2025Article
- Phage-phage competition and biofilms affect interactions between two virulent bacteriophages and Pseudomonas aeruginosa.The ISME journal · 2025Article
- Comparative transcriptomes reveal differential effects on host metabolism reprogramming in two different pelagiphage-SAR11 infection systems.ISME communications · 2025Article
- Article
- Profiling the interplay and coevolution ofMicrobiology spectrum · 2024Article
- Transcriptional dynamics during Rhodococcus erythropolis infection with phage WC1.BMC microbiology · 2024Article
- Thinking Phage Innovations Through Evolution and Ecology.PHAGE (New Rochelle, N.Y.) · 2024Review
- Exploring the transcriptional landscape of phage-host interactions using novel high-throughput approaches.Current opinion in microbiology · 2024Review
- Coordinated transcriptional response to environmental stress by a Synechococcus virus.The ISME journal · 2024Article
- Refining the transcriptional landscapes for distinct clades of virulent phages infectingmicroLife · 2024Article
- Anaerobiosis, a neglected factor in phage-bacteria interactions.Applied and environmental microbiology · 2023Article
- Transcriptional Landscapes ofViruses · 2023Review
- The RNA-RNA interactome between a phage and its satellite virus reveals a small RNA that differentially regulates gene expression across both genomes.Molecular microbiology · 2023Article
- Early-Phase Drive to the Precursor Pool: Chloroviruses Dive into the Deep End of Nucleotide Metabolism.Viruses · 2023Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 4 countries.
Funding
Abstract
As interest in the therapeutic and biotechnological potentials of bacteriophages has grown, so has value in understanding their basic biology. However, detailed knowledge of infection cycles has been limited to a small number of model bacteriophages, mostly infecting Escherichia coli. We present here the first analysis coupling data obtained from global next-generation approaches, RNA-Sequencing and metabolomics, to characterize interactions between the virulent bacteriophage PAK_P3 and its host Pseudomonas aeruginosa. We detected a dramatic global depletion of bacterial transcripts coupled with their replacement by viral RNAs over the course of infection, eventually leading to drastic changes in pyrimidine metabolism. This process relies on host machinery hijacking as suggested by the strong up-regulation of one bacterial operon involved in RNA processing. Moreover, we found that RNA-based regulation plays a central role in PAK_P3 lifecycle as antisense transcripts are produced mainly during the early stage of infection and viral small non coding RNAs are massively expressed at the end of infection. This work highlights the prominent role of RNA metabolism in the infection strategy of a bacteriophage belonging to a new characterized sub-family of viruses with promising therapeutic potential.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.