ArticlePLoS neglected tropical diseases2016
Dual Combined Real-Time Reverse Transcription Polymerase Chain Reaction Assay for the Diagnosis of Lyssavirus Infection.
Article in PLoS neglected tropical diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
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- Rabies viral RNA and antibody response after post-exposure prophylaxis: Implications for blood donor deferral.Tropical diseases, travel medicine and vaccines · 2026Article
- Origin, distribution and incidence of human rabies in Côte d'Ivoire from 2013 to 2023.Journal of virus eradication · 2026Article
- Broad geographical circulation of a novel vesiculovirus in bats in the Mediterranean region.PLoS neglected tropical diseases · 2025Article
- Rabies surveillance in Madagascar from 2011 to 2021: can we reach the target?Frontiers in veterinary science · 2023Article
- CRISPR-Based Programmable Nucleic Acid-Binding Protein Technology Can Specifically Detect Fatal Tropical Disease-Causing Pathogens.Journal of tropical medicine · 2022Review
- A recombinase polymerase amplification assay for rapid detection of rabies virus.Scientific reports · 2021Article
- Multi-annual performance evaluation of laboratories in post-mortem diagnosis of animal rabies: Which techniques lead to the most reliable results in practice?PLoS neglected tropical diseases · 2021Article
- Characterization of Novel Rhabdoviruses in Chinese Bats.Viruses · 2021Article
- Transcriptome Profile During Rabies Virus Infection: Identification of Human CXCL16 as a Potential New Viral Target.Frontiers in cellular and infection microbiology · 2021Article
- Enabling animal rabies diagnostic in low-access areas: Sensitivity and specificity of a molecular diagnostic test from cerebral tissue dried on filter paper.PLoS neglected tropical diseases · 2020Article
- Healthcare utilization, provisioning of post-exposure prophylaxis, and estimation of human rabies burden in Madagascar.Vaccine · 2019Article
- Comparison of intra- and inter-host genetic diversity in rabies virus during experimental cross-species transmission.PLoS pathogens · 2019Article
- Evaluation of six TaqMan RT-rtPCR kits on two thermocyclers for the reliable detection of rabies virus RNA.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- The shift in rabies epidemiology in France: time to adjust rabies post-exposure risk assessment.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2018Article
- Multi-site evaluation of the LN34 pan-lyssavirus real-time RT-PCR assay for post-mortem rabies diagnostics.PloS one · 2018Article
- Lyssaviruses and rabies: current conundrums, concerns, contradictions and controversies.F1000Research · 2017Review
- Validation of a Rapid Rabies Diagnostic Tool for Field Surveillance in Developing Countries.PLoS neglected tropical diseases · 2016Article
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The definitive diagnosis of lyssavirus infection (including rabies) in animals and humans is based on laboratory confirmation. The reference techniques for post-mortem rabies diagnosis are still based on direct immunofluorescence and virus isolation, but molecular techniques, such as polymerase chain reaction (PCR) based methods, are increasingly being used and now constitute the principal tools for diagnosing rabies in humans and for epidemiological analyses. However, it remains a key challenge to obtain relevant specificity and sensitivity with these techniques while ensuring that the genetic diversity of lyssaviruses does not compromise detection. We developed a dual combined real-time reverse transcription polymerase chain reaction (combo RT-qPCR) method for pan-lyssavirus detection. This method is based on two complementary technologies: a probe-based (TaqMan) RT-qPCR for detecting the RABV species (pan-RABV RT-qPCR) and a second reaction using an intercalating dye (SYBR Green) to detect other lyssavirus species (pan-lyssa RT-qPCR). The performance parameters of this combined assay were evaluated with a large panel of primary animal samples covering almost all the genetic variability encountered at the viral species level, and they extended to almost all lyssavirus species characterized to date. This method was also evaluated for the diagnosis of human rabies on 211 biological samples (positive n = 76 and negative n = 135) including saliva, skin and brain biopsies. It detected all 41 human cases of rabies tested and confirmed the sensitivity and the interest of skin biopsy (91.5%) and saliva (54%) samples for intra-vitam diagnosis of human rabies. Finally, this method was successfully implemented in two rabies reference laboratories in enzootic countries (Cambodia and Morocco). This combined RT-qPCR method constitutes a relevant, useful, validated tool for the diagnosis of rabies in both humans and animals, and represents a promising tool for lyssavirus surveillance.
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