ArticleFrontiers in microbiology2016
Colonization of the Intestinal Tract of the Polyphagous Pest Spodoptera littoralis with the GFP-Tagged Indigenous Gut Bacterium Enterococcus mundtii.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Transmisssion Dynamics of Enterococcus spp. Throughout the Heliconius erato phyllis (Lepidoptera; Nymphalidae) Life Cycle.Environmental microbiology reports · 2026Article
- Maintenance of Gut Microbial Balance via the Kynurenine Pathway Improves Larval Performance and Resistance to Bacillus thuringiensis in Spodoptera exigua.MicrobiologyOpen · 2026Article
- Gut microbiome convergence and functional adaptation underlie the evolution of predation in stink bugs (Heteroptera: Pentatomidae).Microbiome · 2026Article
- Microbial Symbiosis in Lepidoptera: Analyzing the Gut Microbiota for Sustainable Pest Management.Biology · 2025Review
- Host Diet and Species Interact to Shape the Bacterial and Fungal Microbiome in the Regurgitant of Four Spodoptera Species.Microbial ecology · 2025Article
- Parasitoid Calyx Fluid and Venom Affect Bacterial Communities in Their Lepidopteran Host Labial Salivary Glands.Microbial ecology · 2025Article
- KtrB-mediated alkaline adaptation drivesFrontiers in microbiology · 2025Article
- Microbiome analysis of monarch butterflies reveals effects of development and diet.FEMS microbiology ecology · 2024Article
- Gut bacteria of lepidopteran herbivores facilitate digestion of plant toxins.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Diversity analyses of bacterial symbionts in fourFrontiers in cellular and infection microbiology · 2024Article
- Sortase-encoding genes,Frontiers in microbiology · 2024Article
- Diversity and Abundance of Bacterial and Fungal Communities InhabitingMicroorganisms · 2023Article
- Host-Adapted Strains of Spodoptera frugiperda Hold and Share a Core Microbial Community Across the Western Hemisphere.Microbial ecology · 2023Article
- Article
- Variation ofFrontiers in microbiology · 2023Article
- 8-HQA adjusts the number and diversity of bacteria in the gut microbiome ofFrontiers in microbiology · 2023Article
- Impact of transgenerational host switch on gut bacterial assemblage in generalist pest,Frontiers in microbiology · 2023Article
- Host's genetic background determines the outcome of reciprocal faecal transplantation on life-history traits and microbiome composition.Animal microbiome · 2022Article
- CRISPR/Cas9-Mediated Genome Editing forInternational journal of molecular sciences · 2022Article
- Features and Colonization Strategies ofFrontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The alkaline gut of Lepidopterans plays a crucial role in shaping communities of bacteria. Enterococcus mundtii has emerged as one of the predominant gut microorganisms in the gastrointestinal tract of the major agricultural pest, Spodoptera littoralis. Therefore, it was selected as a model bacterium to study its adaptation to harsh alkaline gut conditions in its host insect throughout different stages of development (larvae, pupae, adults, and eggs). To date, the mechanism of bacterial survival in insects' intestinal tract has been unknown. Therefore, we have engineered a GFP-tagged species of bacteria, E. mundtii, to track how it colonizes the intestine of S. littoralis. Three promoters of different strengths were used to control the expression of GFP in E. mundtii. The promoter ermB was the most effective, exhibiting the highest GFP fluorescence intensity, and hence was chosen as our main construct. Our data show that the engineered fluorescent bacteria survived and proliferated in the intestinal tract of the insect at all life stages for up to the second generation following ingestion.
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