ArticleFrontiers in microbiology2016
Rapid Detection of Candida albicans by Polymerase Spiral Reaction Assay in Clinical Blood Samples.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Avian Candidiasis: A Comprehensive Review of Pathogenesis, Diagnosis, and Control.Veterinary sciences · 2026Review
- Sensitive and rapid detection ofFrontiers in cellular and infection microbiology · 2026Article
- Novel Isothermal Reverse Transcription Polymerase Spiral Reaction (RT-PSR) Assay for the Detection of Newcastle Disease Virus in Avian Species.Indian journal of microbiology · 2025Article
- Rapid and simple detection ofFrontiers in cellular and infection microbiology · 2025Article
- Multiplex Loop-Mediated Isothermal Amplification Coupled Lateral Flow Assay for Point-of-Care Detection of Syphilis.Indian journal of microbiology · 2024Article
- Innovative Biosensing Approaches for Swift Identification ofLife (Basel, Switzerland) · 2023Review
- A Novel and Rapid Isothermal Nucleic Acid Based Detection Assay ofIranian journal of public health · 2022Article
- Establishment and application of a rapid visual detection method forBioengineered · 2022Article
- Detection ofMicromachines · 2021Article
- Development of a novel polymerase spiral reaction (PSR) assay for rapid and visual detection ofHeliyon · 2021Article
- Article
- Molecular Diagnosis of Yeast Infections.Current fungal infection reports · 2021Review
- Development of magnetic bead based sample extraction coupled polymerase spiral reaction for rapid on-site detection of Chikungunya virus.Scientific reports · 2020Article
- Rapid and Sensitive Detection of Hepatitis C Virus in Clinical Blood Samples Using Reverse Transcriptase Polymerase Spiral Reaction.Journal of microbiology and biotechnology · 2020Article
- Polymerase Spiral Reaction Assay for Rapid and Real Time Detection of West Nile Virus From Clinical Samples.Frontiers in cellular and infection microbiology · 2020Article
- Reduction, Prevention, and Control ofFrontiers in microbiology · 2020Article
- Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics.PloS one · 2020Article
- Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method.BMC veterinary research · 2019Article
- Establishment and Application of Multiple Cross Displacement Amplification Coupled With Lateral Flow Biosensor (MCDA-LFB) for Visual and Rapid Detection ofFrontiers in cellular and infection microbiology · 2019Article
- [Clinical value of droplet digital PCR in rapid diagnosis of invasive fungal infection in neonates].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2019Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Candida albicans is the most common human yeast pathogen which causes mucosal infections and invasive fungal diseases. Early detection of this pathogen is needed to guide preventative and therapeutic treatment. The aim of this study was to establish a polymerase spiral reaction (PSR) assay that rapidly and accurately detects C. albicans and to assess the clinical applicability of PSR-based diagnostic testing. Internal transcribed spacer 2 (ITS2), a region between 5.8S and 28S fungal ribosomal DNA, was used as the target sequence. Four primers were designed for amplification of ITS2 with the PSR method, which was evaluated using real time turbidity monitoring and visual detection using a pH indicator. Fourteen non-C. albicans yeast strains were negative for detection, which indicated the specificity of PSR assay was 100%. A 10-fold serial dilution of C. albicans genomic DNA was subjected to PSR and conventional polimerase chain reaction (PCR) to compare their sensitivities. The detection limit of PSR was 6.9 pg/μl within 1 h, 10-fold higher than that of PCR (69.0 pg/μl). Blood samples (n = 122) were collected from intensive care unit and hematological patients with proven or suspected C. albicans infection at two hospitals in Beijing, China. Both PSR assay and the culture method were used to analyze the samples. Of the 122 clinical samples, 34 were identified as positive by PSR. The result was consistent with those obtained by the culture method. In conclusion, a novel and effective C. albicans detection assay was developed that has a great potential for clinical screening and point-of-care testing.
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