ArticleGene2016
The synovial microenvironment of osteoarthritic joints alters RNA-seq expression profiles of human primary articular chondrocytes.
Article in Gene, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 21 citations in OpenAlex.
- Protein Response to ACL Injury in Humans Show Early Cartilage Remodeling and Differences by Sex.bioRxiv : the preprint server for biology · 2026Article
- Intra-Articular Delivery of Nanoemulsified Curcumin Ameliorates Joint Degeneration in a Chemically Induced Model of Osteoarthritis.International journal of molecular sciences · 2025Article
- The intersection of aging and estrogen in osteoarthritis.npj women's health · 2025Review
- PTH treatment before cyclic joint loading improves cartilage health and attenuates load-induced osteoarthritis development in mice.Science advances · 2024Article
- Comprehensive bulk and single-cell transcriptome profiling give useful insights into the characteristics of osteoarthritis associated synovial macrophages.Frontiers in immunology · 2022Article
- RNA expression profiling from the liquid fraction of synovial fluid in knee joint osteoarthritis patients.American journal of translational research · 2022Article
- Low-Input RNA-Sequencing in Patients with Cartilage Lesions, Osteoarthritis, and Healthy Cartilage.Cartilage · 2021Article
- Chondrocytes from Osteoarthritis Patients Adopt Distinct Phenotypes in Response to Central TInternational journal of molecular sciences · 2021Article
- Differences in Cytotoxicity of Lidocaine, Ropivacaine, and Bupivacaine on the Viability and Metabolic Activity of Human Adipose-Derived Mesenchymal Stem Cells.American journal of physical medicine & rehabilitation · 2021Article
- Widespread regulation of gene expression by glucocorticoids in chondrocytes from patients with osteoarthritis as determined by RNA-Seq.Arthritis research & therapy · 2020Article
- Article
- Effect of Lidocaine on Viability and Gene Expression of Human Adipose-derived Mesenchymal Stem Cells: An in vitro Study.PM & R : the journal of injury, function, and rehabilitation · 2019Article
- Systematic Analysis of Transcriptomic Profile of Chondrocytes in Osteoarthritic Knee Using Next-Generation Sequencing and Bioinformatics.Journal of clinical medicine · 2018Article
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- Article
- Human Engineered Cartilage and Decellularized Matrix as an Alternative to Animal Osteoarthritis Model.Polymers · 2018Article
- Transcriptional profiling of articular cartilage in a porcine model of early post-traumatic osteoarthritis.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- Article
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 3 countries.
Funding
Abstract
Osteoarthritis (OA) is a disabling degenerative joint disease that prompts pain and has limited treatment options. To permit early diagnosis and treatment of OA, a high resolution mechanistic understanding of human chondrocytes in normal and diseased states is necessary. In this study, we assessed the biological effects of OA-related changes in the synovial microenvironment on chondrocytes embedded within anatomically intact cartilage from joints with different pathological grades by next generation RNA-sequencing (RNA-seq). We determined the transcriptome of primary articular chondrocytes derived from anatomically unaffected knees and ankles, as well as from joints affected by OA. The GALAXY bioinformatics platform was used to facilitate biological interpretations. Comparisons of patient samples by k-means, hierarchical clustering and principal component analyses together reveal that primary chondrocytes exhibit OA grade-related differences in gene expression, including genes involved in cell-adhesion, ECM production and immune response. We conclude that diseased synovial microenvironments in joints with different histopathological OA grades directly alter gene expression in chondrocytes. One ramification of this finding is that anatomically intact cartilage from OA joints is not an ideal source of healthy chondrocytes, nor should these specimens be used to generate a normal baseline for the molecular characterization of diseased joints.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.