ArticleNature communications2016
Engineering biosynthesis of the anticancer alkaloid noscapine in yeast.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
69 citing papers in PubMed, 145 citations in OpenAlex.
- Article
- Biosynthesis of benzylisoquinoline alkaloids and its evolution in plants.Plant communications · 2026Review
- Elucidation of gene clusters underlying withanolide biosynthesis in ashwagandha through yeast metabolic engineering.Nature plants · 2026Article
- Enhancing cross-organelle coordination to advance plant cytochrome P450 functionality in yeast.Science advances · 2025Article
- Phosphite mediated molecular editingChemical science · 2025Article
- Engineering Saccharomyces cerevisiae for medical applications.Microbial cell factories · 2025Review
- Genomic and cell-specific regulation of benzylisoquinoline alkaloid biosynthesis in opium poppy.Journal of experimental botany · 2025Review
- Metabolic engineering of Saccharomyces cerevisiae for chelerythrine biosynthesis.Microbial cell factories · 2024Article
- Cutting-edge plant natural product pathway elucidation.Current opinion in biotechnology · 2024Review
- Discovering Dynamic Plant Enzyme Complexes in Yeast for Kratom Alkaloid Pathway Identification.Angewandte Chemie (International ed. in English) · 2023Article
- Design of Four Small-Molecule-Inducible Systems in the Yeast Chromosome, Applied to Optimize Terpene Biosynthesis.ACS synthetic biology · 2023Article
- Multi-Level Optimization and Strategies in Microbial Biotransformation of Nature Products.Molecules (Basel, Switzerland) · 2023Review
- Leveraging yeast to characterize plant biosynthetic gene clusters.Current opinion in plant biology · 2023Review
- Plant Pyranocoumarins: Description, Biosynthesis, Application.Plants (Basel, Switzerland) · 2022Review
- Biosynthesis of tetrahydropapaverine and semisynthesis of papaverine in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Research Progress on the Synthetic Biology of Botanical Biopesticides.Bioengineering (Basel, Switzerland) · 2022Review
- Engineered Production of Strictosidine and Analogues in Yeast.ACS synthetic biology · 2022Article
- A peroxisomal heterodimeric enzyme is involved in benzaldehyde synthesis in plants.Nature communications · 2022Article
- Assessment of Dry Heating, Water Rinsing, and Baking on Concentrations of the Opium Alkaloid Noscapine in Poppy Seeds.ACS food science & technology · 2022Article
- Removal of lycopene substrate inhibition enables high carotenoid productivity in Yarrowia lipolytica.Nature communications · 2022Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Noscapine is a potential anticancer drug isolated from the opium poppy Papaver somniferum, and genes encoding enzymes responsible for the synthesis of noscapine have been recently discovered to be clustered on the genome of P. somniferum. Here, we reconstitute the noscapine gene cluster in Saccharomyces cerevisiae to achieve the microbial production of noscapine and related pathway intermediates, complementing and extending previous in planta and in vitro investigations. Our work provides structural validation of the secoberberine intermediates and the description of the narcotoline-4'-O-methyltransferase, suggesting this activity is catalysed by a unique heterodimer. We also reconstitute a 14-step biosynthetic pathway of noscapine from the simple alkaloid norlaudanosoline by engineering a yeast strain expressing 16 heterologous plant enzymes, achieving reconstitution of a complex plant pathway in a microbial host. Other engineered yeasts produce previously inaccessible pathway intermediates and a novel derivative, thereby advancing protoberberine and noscapine related drug discovery.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.