ArticleFungal genetics and biology : FG & B2016
Metabolic activity in dormant conidia of Aspergillus niger and developmental changes during conidial outgrowth.
Article in Fungal genetics and biology : FG & B, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 74 citations in OpenAlex.
- A Systematic Method for Analysing Germination of Aspergillus Conidia Using Real-Time Imaging.Journal of basic microbiology · 2026Article
- Bronchoalveolar Lavage Fluid Collection in Invasive Pulmonary Aspergillosis Murine Model for Downstream Proteomic Analysis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Review
- Conserved storage-carbohydrate metabolic modules are rewired during germination ofFrontiers in fungal biology · 2026Article
- Antimicrobial effects and mechanisms of hydrogen sulphide against nail pathogens.Scientific reports · 2025Article
- Suppression of rice blast and tomato late blight by Paraboeremia adianticola producing vulculic acid.Pest management science · 2025Article
- Enhanced Extraction of Polyphenols, Physicochemical Properties, and Microbial Control inMolecules (Basel, Switzerland) · 2025Article
- Natural Variation and the Role of ZnMicroorganisms · 2022Article
- Effective Treatment of Cutaneous Mold Infections by Antimicrobial Blue Light That Is Potentiated by Quinine.The Journal of infectious diseases · 2021Article
- Transcription in fungal conidia before dormancy produces phenotypically variable conidia that maximize survival in different environments.Nature microbiology · 2021Article
- Microbial Air Quality in Healthcare Facilities.International journal of environmental research and public health · 2021Review
- Identifying Conserved GenericJournal of fungi (Basel, Switzerland) · 2021Article
- Abundant Small Protein ICARUS Inside the Cell Wall of Stress-Resistant Ascospores ofJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- Optimisation of Sporosori Purification and Protein Extraction Techniques for the Biotrophic Protozoan Plant PathogenMolecules (Basel, Switzerland) · 2020Article
- Untargeted metabolomics as a hypothesis-generation tool in plant protection product discovery: Highlighting the potential of trehalose and glycerol metabolism of fungal conidiospores as novel targets.Metabolomics : Official journal of the Metabolomic Society · 2020Article
- The Preservative Sorbic Acid Targets Respiration, Explaining the Resistance of Fermentative Spoilage Yeast Species.mSphere · 2020Article
- Proteomic analysis of Aspergillus niger 3.316 under heat stress.MicrobiologyOpen · 2020Article
- Molecular Mechanisms of Conidial Germination inMicrobiology and molecular biology reviews : MMBR · 2020Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The early stages of development of Aspergillus niger conidia during outgrowth were explored by combining genome-wide gene expression analysis (RNAseq), proteomics, Warburg manometry and uptake studies. Resting conidia suspended in water were demonstrated for the first time to be metabolically active as low levels of oxygen uptake and the generation of carbon dioxide were detected, suggesting that low-level respiratory metabolism occurs in conidia for maintenance. Upon triggering of spore germination, generation of CO2 increased dramatically. For a short period, which coincided with mobilisation of the intracellular polyol, trehalose, there was no increase in uptake of O2 indicating that trehalose was metabolised by fermentation. Data from genome-wide mRNA profiling showed the presence of transcripts associated with fermentative and respiratory metabolism in resting conidia. Following triggering of conidial outgrowth, there was a clear switch to respiration after 25min, confirmed by cyanide inhibition. No effect of SHAM, salicylhydroxamic acid, on respiration suggests electron flow via cytochrome c oxidase. Glucose entry into spores was not detectable before 1h after triggering germination. The impact of sorbic acid on germination was examined and we showed that it inhibits glucose uptake. O2 uptake was also inhibited, delaying the onset of respiration and extending the period of fermentation. In conclusion, we show that conidia suspended in water are not completely dormant and that conidial outgrowth involves fermentative metabolism that precedes respiration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.