ArticleJournal of neuroscience methods2016
Brain tissue stiffness is a sensitive marker for acidosis.
Article in Journal of neuroscience methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
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- Review of the Brain's Behaviour after Injury and Disease for Its Application in an Agent-Based Model (ABM).Biomimetics (Basel, Switzerland) · 2024Review
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- Nanomechanical and Morphological AFM Mapping of Normal Tissues and Tumors on Live Brain Slices Using Specially Designed Embedding Matrix and Laser-Shaped Cantilevers.Biomedicines · 2022Article
- Mechanical Properties of the Extracellular Environment of Human Brain Cells Drive the Effectiveness of Drugs in Fighting Central Nervous System Cancers.Brain sciences · 2022Review
- Atomic Force Microscope Nanoindentation Analysis of Diffuse Astrocytic Tumor Elasticity: Relation with Tumor Histopathology.Cancers · 2021Article
- The Cytoskeletal Elements MAP2 and NF-L Show Substantial Alterations in Different Stroke Models While Elevated Serum Levels Highlight Especially MAP2 as a Sensitive Biomarker in Stroke Patients.Molecular neurobiology · 2021Article
- Establishing an Adult Mouse Brain Hippocampal Organotypic Slice Culture System that Allows for Tracing and Pharmacological Manipulation ofBio-protocol · 2021Article
- Spatial mapping of the collagen distribution in human and mouse tissues by force volume atomic force microscopy.Scientific reports · 2020Article
- Substrate-Dependent Galvanotaxis of Directly Reprogrammed Human Neural Precursor Cells.Bioelectricity · 2020Article
- Zebrafish Spinal Cord Repair Is Accompanied by Transient Tissue Stiffening.Biophysical journal · 2020Article
- Mechanical regulation of oligodendrocyte biology.Neuroscience letters · 2020Review
- Nanomechanics and Histopathology as Diagnostic Tools to Characterize Freshly Removed Human Brain Tumors.International journal of nanomedicine · 2020Article
- Mechanics of Brain Tissues Studied by Atomic Force Microscopy: A Perspective.Frontiers in neuroscience · 2019Review
- Mechanical Mapping of Spinal Cord Growth and Repair in Living Zebrafish Larvae by Brillouin Imaging.Biophysical journal · 2018Article
- The role of cell body density in ruminant retina mechanics assessed by atomic force and Brillouin microscopy.Physical biology · 2017Article
- Appropriate Scaffold Selection for CNS Tissue Engineering.Avicenna journal of medical biotechnologyReview
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundCarbon dioxide overdose is frequently used to cull rodents for tissue harvesting. However, this treatment may lead to respiratory acidosis, which potentially could change the properties of the investigated tissue. NEW
methodMechanical tissue properties often change in pathological conditions and may thus offer a sensitive generic readout for changes in biological tissues with clinical relevance. In this study, we performed force-indentation measurements with an atomic force microscope on acute cerebellar slices from adult rats to test if brain tissue undergoes changes following overexposure to CO2 compared to other methods of euthanasia.
resultsThe pH significantly decreased in brain tissue of animals exposed to CO2. Concomitant with the drop in pH, cerebellar grey matter significantly stiffened. Tissue stiffening was reproduced by incubation of acute cerebellar slices in acidic medium. COMPARISON WITH EXISTING
methodsTissue stiffness provides an early, generic indicator for pathophysiological changes in the CNS. Atomic force microscopy offers unprecedented high spatial resolution to detect such changes.
conclusionsOur results indicate that the stiffness particularly of grey matter strongly correlates with changes of the pH in the cerebellum. Furthermore, the method of tissue harvesting and preparation may not only change tissue stiffness but very likely also other physiologically relevant parameters, highlighting the importance of appropriate sample preparation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.