Evidence map›Paper›PMID 27377583›Full record

ArticleDevelopmental and comparative immunology2016

Identification of secreted and membrane-bound bat immunoglobulin using a Microchiropteran-specific mouse monoclonal antibody.

William T Lee, Derek D Jones, Jennifer L Yates, Gary M Winslow, April D Davis, Robert J Rudd, Christopher T Barron, Cailyn Cowan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental and comparative immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
4.4field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

William T LeeThe Department of Biomedical Sciences, The School of Public Health, The University at Albany, Albany, NY, 12201-0509, USA; The Laboratory of Immunology, The Wadsworth Center, New York State Department of Health, Albany, NY, 12201-2002, USA. Electronic address: William.lee@health.ny.gov.
Derek D JonesThe Department of Biomedical Sciences, The School of Public Health, The University at Albany, Albany, NY, 12201-0509, USA.
Jennifer L YatesThe Department of Biomedical Sciences, The School of Public Health, The University at Albany, Albany, NY, 12201-0509, USA.
Gary M WinslowThe Department of Biomedical Sciences, The School of Public Health, The University at Albany, Albany, NY, 12201-0509, USA; The Laboratory of Immunology, The Wadsworth Center, New York State Department of Health, Albany, NY, 12201-2002, USA.
April D DavisThe Laboratory of Immunology, The Wadsworth Center, New York State Department of Health, Albany, NY, 12201-2002, USA.
Robert J RuddThe Laboratory of Immunology, The Wadsworth Center, New York State Department of Health, Albany, NY, 12201-2002, USA.
Christopher T BarronThe Laboratory of Immunology, The Wadsworth Center, New York State Department of Health, Albany, NY, 12201-2002, USA.
Cailyn CowanThe Department of Biomedical Sciences, The School of Public Health, The University at Albany, Albany, NY, 12201-0509, USA.
New York State Department of Health · USUniversity at Albany, State University of New York · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bat immunity has received increasing attention because some bat species are being decimated by the fungal disease, White Nose Syndrome, while other species are potential reservoirs of zoonotic viruses. Identifying specific immune processes requires new specific tools and reagents. In this study, we describe a new mouse monoclonal antibody (mAb) reactive with Eptesicus fuscus immunoglobulins. The epitope recognized by mAb BT1-4F10 was localized to immunoglobulin light (lambda) chains; hence, the mAb recognized serum immunoglobulins and B lymphocytes. The BT1-4F10 epitope appeared to be restricted to Microchiropteran immunoglobulins and absent from Megachiropteran immunoglobulins. Analyses of sera and other E. fuscus fluids showed that most, if not all, secreted immunoglobulins utilized lambda light chains. Finally, mAb BT1-4F10 permitted the identification of B cell follicles in splenic white pulp. This Microchiropteran-specific mAb has potential utility in seroassays; hence, this reagent may have both basic and practical applications for studying immune process.

Indexed as

AnimalsAntibodies, FungalAntibodies, MonoclonalB-LymphocytesCell LineCell SeparationChiropteraEpitope MappingFlow CytometryImmunoglobulin lambda-ChainsImmunophenotypingMiceMicroscopyMycosesZoonosesAntibodies, FungalAntibodies, MonoclonalImmunoglobulin lambda-ChainsBatsB lymphocytesImmunoglobulinsMicrochiropteraMonoclonal antibody

Identifiers

PMID27377583
PMCPMC7172696
OpenAlexW2465282337

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.