ArticleAdvanced healthcare materials2016
Collagen Substrate Stiffness Anisotropy Affects Cellular Elongation, Nuclear Shape, and Stem Cell Fate toward Anisotropic Tissue Lineage.
Article in Advanced healthcare materials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 64 citations in OpenAlex.
- Rational design of immunological programs for functional regenerative niches.Materials today. Bio · 2026Review
- The mechanobiology of extracellular matrix: a focus on thrombospondins.Cell communication and signaling : CCS · 2025Review
- Evenly Distributed Microporous Structure and E7 Peptide Functionalization Synergistically Accelerate Osteogenesis and Angiogenesis in Engineered Periosteum.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Stimuli-Responsive Substrates to Control the Immunomodulatory Potential of Stromal Cells.Biomacromolecules · 2024Review
- Stiffness anisotropy coordinates supracellular contractility driving long-range myotube-ECM alignment.Science advances · 2024Article
- Molecular-scale substrate anisotropy, crowding and division drive collective behaviours in cell monolayers.Journal of the Royal Society, Interface · 2023Article
- Recent Advances in Tissue-Engineered Cardiac Scaffolds-The Progress and Gap in Mimicking Native Myocardium Mechanical Behaviors.Journal of functional biomaterials · 2023Review
- Cardiac-Adaptive Conductive Hydrogel Patch Enabling Construction of Mechanical-Electrical Anisotropic Microenvironment for Heart Repair.Research (Washington, D.C.) · 2023Article
- A preliminary evaluation of in vivo response to a filament-wound macroporous collagen midurethral sling in an ovine model.Journal of biomedical materials research. Part B, Applied biomaterials · 2022Article
- Toward Hierarchical Assembly of Aligned Cell Sheets into a Conical Cardiac Ventricle Using Microfabricated Elastomers.Advanced biology · 2022Article
- An overview of substrate stiffness guided cellular response and its applications in tissue regeneration.Bioactive materials · 2022Review
- Mesenchymal Stem Cell Delivery via Topographically Tenoinductive Collagen Biotextile Enhances Regeneration of Segmental Tendon Defects.The American journal of sports medicine · 2022Article
- Tissue Engineering Strategies to Increase Osteochondral Regeneration of Stem Cells; a Close Look at Different Modalities.Stem cell reviews and reports · 2021Review
- Microengineered 3D Collagen Gels with Independently Tunable Fiber Anisotropy and Directionality.Advanced materials technologies · 2021Article
- Long-range mechanical signaling in biological systems.Soft matter · 2021Review
- Magnetic Micromanipulation forResearch (Washington, D.C.) · 2020Article
- Electrobiofabrication: electrically based fabrication with biologically derived materials.Biofabrication · 2019Review
- Atomic force acoustic microscopy reveals the influence of substrate stiffness and topography on cell behavior.Beilstein journal of nanotechnology · 2019Article
- Thrombospondin-4 in tissue remodeling.Matrix biology : journal of the International Society for Matrix Biology · 2019Review
- Tenogenesis of Decellularized Porcine Achilles Tendon Matrix Reseeded with Human Tenocytes in the Nude Mice Xenograft Model.International journal of molecular sciences · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Rigidity of substrates plays an important role in stem cell fate. Studies are commonly carried out on isotropically stiff substrate or substrates with unidirectional stiffness gradients. However, many native tissues are anisotropically stiff and it is unknown whether controlled presentation of stiff and compliant material axes on the same substrate governs cytoskeletal and nuclear morphology, as well as stem cell differentiation. In this study, electrocompacted collagen sheets are stretched to varying degrees to tune the stiffness anisotropy (SA) in the range of 1 to 8, resulting in stiff and compliant material axes orthogonal to each other. The cytoskeletal aspect ratio increased with increasing SA by about fourfold. Such elongation was absent on cellulose acetate replicas of aligned collagen surfaces indicating that the elongation was not driven by surface topography. Mesenchymal stem cells (MSCs) seeded on varying anisotropy sheets displayed a dose-dependent upregulation of tendon-related markers such as Mohawk and Scleraxis. After 21 d of culture, highly anisotropic sheets induced greater levels of production of type-I, type-III collagen, and thrombospondin-4. Therefore, SA has direct effects on MSC differentiation. These findings may also have ramifications of stem cell fate on other anisotropically stiff tissues, such as skeletal/cardiac muscles, ligaments, and bone.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.