ArticleScientific reports2016
Identification, Characterization, and X-ray Crystallographic Analysis of a Novel Type of Mannose-Specific Lectin CGL1 from the Pacific Oyster Crassostrea gigas.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- A novel intracellular signaling pathway elicited by DM9CP-6 regulates immune responses in oysters.Cell communication and signaling : CCS · 2025Article
- Functional Diversity of Novel Lectins with Unique Structural Features in Marine Animals.Cells · 2023Review
- CgDM9CP-5-Integrin-MAPK Pathway Regulates the Production of CgIL-17s and Cgdefensins in the Pacific Oyster, Crassostrea gigas.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Utilizing an artificial intelligence system to build the digital structural proteome of reef-building corals.GigaScience · 2022Article
- Echinococcus granulosus Protoscolex DM9 Protein Shows High Potential for Serodiagnosis of Alveolar Echinococcosis.The Korean journal of parasitology · 2022Article
- Evaluation of Glycan-Binding Specificity by Glycoconjugate Microarray with an Evanescent-Field Fluorescence Detection System.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Natterin Proteins Diversity: A Review on Phylogeny, Structure, and Immune Function.Toxins · 2021Review
- A Comprehensive Phylogenetic and Bioinformatics Survey of Lectins in the Fungal Kingdom.Journal of fungi (Basel, Switzerland) · 2021Article
- Agglutination Activity of Fasciola gigantica DM9-1, a Mannose-Binding Lectin.The Korean journal of parasitology · 2021Article
- LectomeXplore, an update of UniLectin for the discovery of carbohydrate-binding proteins based on a new lectin classification.Nucleic acids research · 2021Article
- A GM1b/asialo-GM1 oligosaccharide-binding R-type lectin from purplish bifurcate mussels Mytilisepta virgata and its effect on MAP kinases.The FEBS journal · 2020Article
- Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific OysterFrontiers in immunology · 2020Article
- Horizontal transfer of a natterin-like toxin encoding gene within the holobiont of the reef building coralJournal of venom research · 2020Article
- Lectin engineering: the possible and the actual.Interface focus · 2019Review
- Novel CaProtein science : a publication of the Protein Society · 2019Article
- Identification, Characterization, and X-ray Crystallographic Analysis of a Novel Type of Lectin AJLec from the Sea Anemone Anthopleura japonica.Scientific reports · 2018Article
- Pathogen-Derived Carbohydrate Recognition in Molluscs Immune Defense.International journal of molecular sciences · 2018Review
- DM9 Domain Containing Protein Functions As a Pattern Recognition Receptor with Broad Microbial Recognition Spectrum.Frontiers in immunology · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel mannose-specific lectin, named CGL1 (15.5 kDa), was isolated from the oyster Crassostrea gigas. Characterization of CGL1 involved isothermal titration calorimetry (ITC), glycoconjugate microarray, and frontal affinity chromatography (FAC). This analysis revealed that CGL1 has strict specificity for the mannose monomer and for high mannose-type N-glycans (HMTGs). Primary structure of CGL1 did not show any homology with known lectins but did show homology with proteins of the natterin family. Crystal structure of the CGL1 revealed a unique homodimer in which each protomer was composed of 2 domains related by a pseudo two-fold axis. Complex structures of CGL1 with mannose molecules showed that residues have 8 hydrogen bond interactions with O1, O2, O3, O4, and O5 hydroxyl groups of mannose. The complex interactions that are not observed with other mannose-binding lectins revealed the structural basis for the strict specificity for mannose. These characteristics of CGL1 may be helpful as a research tool and for clinical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.