ArticleScientific reports2016
Proteome-wide measurement of non-canonical bacterial mistranslation by quantitative mass spectrometry of protein modifications.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Mistranslation from an endogenous tRNA variant in human pan-genome cell lines.Nucleic acids research · 2026Article
- Mechanisms and Delivery of tRNA Therapeutics.Chemical reviews · 2024Review
- Phosphoproteome Dynamics of Streptomyces rimosus during Submerged Growth and Antibiotic Production.mSystems · 2022Article
- A pair of isoleucyl-tRNA synthetases in Bacilli fulfills complementary roles to keep fast translation and provide antibiotic resistance.Protein science : a publication of the Protein Society · 2022Article
- A novel fluorescent reporter sensitive to serine mis-incorporation.RNA biology · 2022Article
- Misincorporation Proteomics Technologies: A Review.Proteomes · 2021Review
- Open Database Searching Enables the Identification and Comparison of Bacterial Glycoproteomes without Defining Glycan Compositions Prior to Searching.Molecular & cellular proteomics : MCP · 2020Article
- Exploiting evolutionary trade-offs for posttreatment management of drug-resistant populations.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Visualisation tools for dependent peptide searches to support the exploration of in vitro protein modifications.PloS one · 2020Article
- Broad range of missense error frequencies in cellular proteins.Nucleic acids research · 2019Article
- Loss of protein synthesis quality control in host-restricted organisms.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- mRNA Translation Gone Awry: Translation Fidelity and Neurological Disease.Trends in genetics : TIG · 2018Review
- MS-READ: Quantitative measurement of amino acid incorporation.Biochimica et biophysica acta. General subjects · 2017Article
- Self-protective responses to norvaline-induced stress in a leucyl-tRNA synthetase editing-deficient yeast strain.Nucleic acids research · 2017Article
- Mass spectrometric identification of phenol-soluble modulins in the ATCC® 43300 standard strain of methicillin-resistant Staphylococcus aureus harboring two distinct phenotypes.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- Discovery and Investigation of Natural Editing Function against Artificial Amino Acids in Protein Translation.ACS central science · 2017Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genetic code is virtually universal in biology and was likely established before the advent of cellular life. The extent to which mistranslation occurs is poorly understood and presents a fundamental question in basic research and production of recombinant proteins. Here we used shotgun proteomics combined with unbiased protein modification analysis to quantitatively analyze in vivo mistranslation in an E. coli strain with a defect in the editing mechanism of leucyl-tRNA synthetase. We detected the misincorporation of a non-proteinogenic amino acid norvaline on 10% of all measured leucine residues under microaerobic conditions and revealed preferential deployment of a tRNA(Leu)(CAG) isoacceptor during norvaline misincorporation. The strain with the norvalylated proteome demonstrated a substantial reduction in cell fitness under both prolonged aerobic and microaerobic cultivation. Unlike norvaline, isoleucine did not substitute for leucine even under harsh error-prone conditions. Our study introduces shotgun proteomics as a powerful tool in quantitative analysis of mistranslation.
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