Evidence map›Paper›PMID 27376968›Full record

ArticleProtein science : a publication of the Protein Society2016

The yeast transcription elongation factor Spt4/5 is a sequence-specific RNA binding protein.

Amanda J Blythe, Berra Yazar-Klosinski, Michael W Webster, Eefei Chen, Marylène Vandevenne, Katerina Bendak, Joel P Mackay, Grant A Hartzog, Alice Vrielink

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein science : a publication of the Protein Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Amanda J BlytheSchool of Chemistry and Biochemistry, University of Western Australia, Crawley, Western Australia, 6009, Australia.
Berra Yazar-KlosinskiDepartment of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, California, 95064.
Michael W WebsterSchool of Molecular Bioscience, University of Sydney, Sydney, New South Wales, 2006, Australia.
Eefei ChenDepartment of Chemistry and Biochemistry, University of California, Santa Cruz, California, 95064.
Marylène VandevenneSchool of Molecular Bioscience, University of Sydney, Sydney, New South Wales, 2006, Australia.
Katerina BendakSchool of Molecular Bioscience, University of Sydney, Sydney, New South Wales, 2006, Australia.
Joel P MackaySchool of Molecular Bioscience, University of Sydney, Sydney, New South Wales, 2006, Australia.
Grant A HartzogDepartment of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, California, 95064.
Alice VrielinkSchool of Chemistry and Biochemistry, University of Western Australia, Crawley, Western Australia, 6009, Australia.
University of Sydney · AUUniversity of California, Santa Cruz · USUniversity of Western Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The heterodimeric transcription elongation factor Spt4/Spt5 (Spt4/5) tightly associates with RNAPII to regulate both transcriptional elongation and co-transcriptional pre-mRNA processing; however, the mechanisms by which Spt4/5 acts are poorly understood. Recent studies of the human and Drosophila Spt4/5 complexes indicate that they can bind nucleic acids in vitro. We demonstrate here that yeast Spt4/5 can bind in a sequence-specific manner to single stranded RNA containing AAN repeats. Furthermore, we show that the major protein determinants for RNA-binding are Spt4 together with the NGN domain of Spt5 and that the KOW domains are not required for RNA recognition. These findings attribute a new function to a domain of Spt4/5 that associates directly with RNAPII, making significant steps towards elucidating the mechanism behind transcriptional control by Spt4/5.

Indexed as

AnimalsChromosomal Proteins, Non-HistoneDrosophila melanogasterHumansNuclear ProteinsProtein DomainsRNA-Binding ProteinsRNA Polymerase IISaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscriptional Elongation FactorsChromosomal Proteins, Non-HistoneNuclear ProteinsRNA-Binding ProteinsRNA Polymerase IISaccharomyces cerevisiae ProteinsSPT4 protein, S cerevisiaeSPT5 transcriptional elongation factorTranscriptional Elongation FactorsRNA bindingRNA polymeraseSELEXSpt4/5transcription elongationtranscription elongation factor

Identifiers

PMID27376968
PMCPMC5338239
OpenAlexW2463989403

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.