Evidence map›Paper›PMID 27376799›Full record

ArticleJournal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban2016

Thymosin β4 impeded murine stem cell proliferation with an intact cardiovascular differentiation.

Li Nie, Shi-Jun Gao, Ya-Nan Zhao, Jacob Masika, Hong-Yan Luo, Xin-Wu Hu, Liang-Pin Zhang, Ying Zeng, Jürgen Hescheler, Hua-Min Liang

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 3 countries.

Li NieDepartment of Physiology, Chinese-German Stem Cell Center, the Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation, Hubei Province, Huazhong University of Science and Technology, Wuhan, 430030, China.
Shi-Jun GaoDepartment of Physiology, Chinese-German Stem Cell Center, the Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation, Hubei Province, Huazhong University of Science and Technology, Wuhan, 430030, China.
Ya-Nan ZhaoDepartment of Physiology, Chinese-German Stem Cell Center, the Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation, Hubei Province, Huazhong University of Science and Technology, Wuhan, 430030, China.
Jacob MasikaDepartment of Physiology, Chinese-German Stem Cell Center, the Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation, Hubei Province, Huazhong University of Science and Technology, Wuhan, 430030, China.
Hong-Yan LuoDepartment of Physiology, Chinese-German Stem Cell Center, the Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation, Hubei Province, Huazhong University of Science and Technology, Wuhan, 430030, China.
Xin-Wu HuDepartment of Physiology, Chinese-German Stem Cell Center, the Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation, Hubei Province, Huazhong University of Science and Technology, Wuhan, 430030, China.
Liang-Pin ZhangDepartment of Physiology, Chinese-German Stem Cell Center, the Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation, Hubei Province, Huazhong University of Science and Technology, Wuhan, 430030, China.
Ying ZengUnion Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China.
Jürgen HeschelerInstitute of Neurophysiology, University of Cologne, Robert Koch str. 39, Cologne, 50931, Germany.
Hua-Min LiangDepartment of Physiology, Chinese-German Stem Cell Center, the Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation, Hubei Province, Huazhong University of Science and Technology, Wuhan, 430030, China. lianghuamin76@163.com.
Huazhong University of Science and Technology · CNHubei University of Chinese Medicine · CNUniversity of Cologne · DEWuhan Institute of Bioengineering · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Thymosin β4 (Tβ4) is a key factor in cardiac development, growth, disease, epicardial integrity, blood vessel formation and has cardio-protective properties. However, its role in murine embryonic stem cells (mESCs) proliferation and cardiovascular differentiation remains unclear. Thus we aimed to elucidate the influence of Tβ4 on mESCs. Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes. It was found that Tβ4 decreased mESCs proliferation in a partial dose-dependent manner and the expression of cell cycle regulatory genes c-myc, c-fos and c-jun. However, mESCs self-renewal markers Oct4 and Nanog were elevated, indicating the maintenance of self-renewal ability in these mESCs. Phosphorylation of STAT3 and Akt was inhibited by Tβ4 while the expression of RAS and phosphorylation of ERK were enhanced. No significant difference was found in BMP2/BMP4 or their downstream protein smad. Wnt3 and Wnt11 were remarkably decreased by Tβ4 with upregulation of Tcf3 and constant β-catenin. Under mESCs differentiation, Tβ4 treatment did not change the expression of cardiovascular cell markers α-MHC, PECAM, and α-SMA. Neither the electrophysiological properties of mESCs-derived cardiomyocytes nor the hormonal regulation by Iso/Cch was affected by Tβ4. In conclusion, Tβ4 suppressed mESCs proliferation by affecting the activity of STAT3, Akt, ERK and Wnt pathways. However, Tβ4 did not influence the in vitro cardiovascular differentiation.

Indexed as

AnimalsCell CycleCell DifferentiationCell MovementCell ProliferationDose-Response Relationship, DrugExtracellular Signal-Regulated MAP KinasesGene Expression RegulationJNK Mitogen-Activated Protein KinasesMiceMouse Embryonic Stem CellsMyocytes, CardiacNanog Homeobox ProteinOctamer Transcription Factor-3Patch-Clamp TechniquesPrimary Cell CultureExtracellular Signal-Regulated MAP KinasesJNK Mitogen-Activated Protein KinasesMyc protein, mouseNanog Homeobox ProteinNanog protein, mouseOctamer Transcription Factor-3Pou5f1 protein, mouseProto-Oncogene Proteins c-aktProto-Oncogene Proteins c-fosProto-Oncogene Proteins c-mycStat3 protein, mouseSTAT3 Transcription FactorThymosinThymosin beta(4)cardiogenesismurine embryonic stem cellspatch clampproliferationthymosin β4

Identifiers

PMID27376799
OpenAlexW2464431775

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.