ArticleNature structural & molecular biology2016
The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease.
Article in Nature structural & molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- PXL: a nucleic acid-binding module of the promyelocytic leukemia protein.Nucleic acids research · 2026Article
- PXL: a Nucleic Acid-Binding Module of Promyelocytic Leukemia Protein.bioRxiv : the preprint server for biology · 2026Article
- The role of secondary structures in the functioning of 3' untranslated regions of mRNA: A review of functions of 3' UTRs' secondary structures and hypothetical involvement of secondary structures in cytoplasmic polyadenylation in Drosophila.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Optimal RNA binding by Egalitarian, a Dynein cargo adaptor, is critical for maintaining oocyte fate inRNA biology · 2021Article
- Modulating the bicoid gradient in space and time.Hereditas · 2021Article
- Repeated Evolution of Inactive Pseudonucleases in a Fungal Branch of the Dis3/RNase II Family of Nucleases.Molecular biology and evolution · 2021Article
- Structure-function analysis of microRNA 3'-end trimming by Nibbler.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Patterning the Drosophila embryo: A paradigm for RNA-based developmental genetic regulation.Wiley interdisciplinary reviews. RNA · 2020Review
- An Interaction Network of RNA-Binding Proteins Involved inMolecular & cellular proteomics : MCP · 2020Article
- Mechanisms and Regulation of RNA Condensation in RNP Granule Formation.Trends in biochemical sciences · 2020Review
- A Novel Splice Variant of the Masculinizing GeneBiomolecules · 2019Article
- mRNA localization in metazoans: A structural perspective.RNA biology · 2017Review
- Evolutionary coincidence of adaptive changes in exuperantia and the emergence of bicoid in Cyclorrhapha (Diptera).Development genes and evolution · 2017Article
- Article
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7 authors at 4 institutions in 1 country.
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Abstract
Anterior patterning in Drosophila is mediated by the localization of bicoid (bcd) mRNA at the anterior pole of the oocyte. Exuperantia (Exu) is a putative exonuclease (EXO) associated with bcd and required for its localization. We present the crystal structure of Exu, which reveals a dimeric assembly with each monomer consisting of a 3'-5' EXO-like domain and a sterile alpha motif (SAM)-like domain. The catalytic site is degenerate and inactive. Instead, the EXO-like domain mediates dimerization and RNA binding. We show that Exu binds RNA directly in vitro, that the SAM-like domain is required for RNA binding activity and that Exu binds a structured element present in the bcd 3' untranslated region with high affinity. Through structure-guided mutagenesis, we show that Exu dimerization is essential for bcd localization. Our data demonstrate that Exu is a noncanonical RNA-binding protein with EXO-SAM-like domain architecture that interacts with its target RNA as a homodimer.
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