ArticleCellular microbiology2017
Long-term live-cell imaging reveals new roles for Salmonella effector proteins SseG and SteA.
Article in Cellular microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- TRIM21 induces selective autophagy of viruses and bacteria.Molecular cell · 2026Article
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- Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Critical role ofApplied and environmental microbiology · 2024Article
- Review
- TheMicrobiology (Reading, England) · 2023Article
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- Monocyte-Derived Chicken Macrophages Exposed toMicroorganisms · 2023Article
- Speaking the host language: howMicrobiology (Reading, England) · 2023Review
- Article
- CIEGAN: A Deep Learning Tool for Cell Image Enhancement.Frontiers in genetics · 2022Article
- For the Greater (Bacterial) Good: Heterogeneous Expression of Energetically Costly Virulence Factors.Infection and immunity · 2020Review
- Clofazimine Reduces the Survival ofACS infectious diseases · 2020Article
- Proteomic Analysis ofMolecular & cellular proteomics : MCP · 2020Article
- Rice aquaporin PIP1;3 and harpin Hpa1 of bacterial blight pathogen cooperate in a type III effector translocation.Journal of experimental botany · 2019Article
- Article
- Article
- A Multicolor Split-Fluorescent Protein Approach to Visualize Listeria Protein Secretion in Infection.Biophysical journal · 2018Article
- TheThe Journal of biological chemistry · 2018Article
- A cell-based infection assay identifies efflux pump modulators that reduce bacterial intracellular load.PLoS pathogens · 2018Article
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Authors and funding
9 authors.
Funding
Abstract
Salmonella Typhimurium is an intracellular bacterial pathogen that infects both epithelial cells and macrophages. Salmonella effector proteins, which are translocated into the host cell and manipulate host cell components, control the ability to replicate and/or survive in host cells. Due to the complexity and heterogeneity of Salmonella infections, there is growing recognition of the need for single-cell and live-cell imaging approaches to identify and characterize the diversity of cellular phenotypes and how they evolve over time. Here, we establish a pipeline for long-term (17 h) live-cell imaging of infected cells and subsequent image analysis methods. We apply this pipeline to track bacterial replication within the Salmonella-containing vacuole in epithelial cells, quantify vacuolar replication versus survival in macrophages and investigate the role of individual effector proteins in mediating these parameters. This approach revealed that dispersed bacteria can coalesce at later stages of infection, that the effector protein SseG influences the propensity for cytosolic hyper-replication in epithelial cells, and that while SteA only has a subtle effect on vacuolar replication in epithelial cells, it has a profound impact on infection parameters in immunocompetent macrophages, suggesting differential roles for effector proteins in different infection models.
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