Evidence map›Paper›PMID 27376336›Full record

ArticlePathogens (Basel, Switzerland)2016

Simultaneous Quantification and Differentiation of Streptococcus suis Serotypes 2 and 9 by Quantitative Real-Time PCR, Evaluated in Tonsillar and Nasal Samples of Pigs.

Niels Dekker, Ineke Daemen, Koen Verstappen, Astrid de Greeff, Hilde Smith, Birgitta Duim

Open access · goldAbstract read
In one paragraph

Article in Pathogens (Basel, Switzerland), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.5field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
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  7. Why Veterinarians (Do Not) Adhere to the Clinical PracticeAntibiotics (Basel, Switzerland) · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Niels DekkerFaculty of Veterinary Medicine, Department of Farm Animal Health, Utrecht University, P.O. Box 80.151, 3508 TD Utrecht, The Netherlands. c.n.t.dekker@uu.nl.
Ineke DaemenFaculty of Veterinary Medicine, Department of Farm Animal Health, Utrecht University, P.O. Box 80.151, 3508 TD Utrecht, The Netherlands. a.j.j.m.daemen@uu.nl.
Koen VerstappenFaculty of Veterinary Medicine, Department of Infectious Diseases and Immunology, Utrecht University, P.O. Box 80.165, 3508 TD Utrecht, The Netherlands. k.verstappen@uu.nl.
Astrid de GreeffCentral Veterinary Institute of Wageningen UR, P.O. Box 65, 8200 AB Lelystad, The Netherlands. astrid.degreeff@wur.nl.
Hilde SmithCentral Veterinary Institute of Wageningen UR, P.O. Box 65, 8200 AB Lelystad, The Netherlands. hilde.smith@wur.nl.
Birgitta DuimFaculty of Veterinary Medicine, Department of Infectious Diseases and Immunology, Utrecht University, P.O. Box 80.165, 3508 TD Utrecht, The Netherlands. b.duim@uu.nl.
Utrecht University · NLWageningen University & Research · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Invasive Streptococcus suis (S. suis) infections in pigs are often associated with serotypes 2 and 9. Mucosal sites of healthy pigs can be colonized with these serotypes, often multiple serotypes per pig. To unravel the contribution of these serotypes in pathogenesis and epidemiology, simultaneous quantification of serotypes is needed. A quantitative real-time PCR (qPCR) targeting cps2J (serotypes 2 and 1/2) and cps9H (serotype 9) was evaluated with nasal and tonsillar samples from S. suis exposed pigs. qPCR specifically detected serotypes in all pig samples. The serotypes loads in pig samples estimated by qPCR showed, except for serotype 9 in tonsillar samples (correlation coefficient = 0.25), moderate to strong correlation with loads detected by culture (correlation coefficient > 0.65), and also in pigs exposed to both serotypes (correlation coefficient > 0.75). This qPCR is suitable for simultaneous differentiation and quantification of important S. suis serotypes.

Indexed as

cpsnosepigqPCRserotype 2serotype 9S. suisStreptococcus suistonsil

Identifiers

PMID27376336
PMCPMC5039426
OpenAlexW2464902634

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.