Evidence map›Paper›PMID 27376020›Full record

ArticleWorld journal of methodology2016

Rat model of cholelithiasis with human gallstones implanted in cholestasis-induced virtual gallbladder.

Marlein Miranda Cona, Yewei Liu, Ting Yin, Yuanbo Feng, Feng Chen, Stefaan Mulier, Yue Li, Jian Zhang, Raymond Oyen, Yicheng Ni

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of methodology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 3 countries.

Marlein Miranda ConaMarlein Miranda Cona, Yewei Liu, Ting Yin, Yuanbo Feng, Feng Chen, Stefaan Mulier, Raymond Oyen, Yicheng Ni, Department of Imaging and Pathology, Faculty of Medicine, Biomedical Sciences Group, KU Leuven, 3000 Leuven, Belgium.
Yewei LiuMarlein Miranda Cona, Yewei Liu, Ting Yin, Yuanbo Feng, Feng Chen, Stefaan Mulier, Raymond Oyen, Yicheng Ni, Department of Imaging and Pathology, Faculty of Medicine, Biomedical Sciences Group, KU Leuven, 3000 Leuven, Belgium.
Ting YinMarlein Miranda Cona, Yewei Liu, Ting Yin, Yuanbo Feng, Feng Chen, Stefaan Mulier, Raymond Oyen, Yicheng Ni, Department of Imaging and Pathology, Faculty of Medicine, Biomedical Sciences Group, KU Leuven, 3000 Leuven, Belgium.
Yuanbo FengMarlein Miranda Cona, Yewei Liu, Ting Yin, Yuanbo Feng, Feng Chen, Stefaan Mulier, Raymond Oyen, Yicheng Ni, Department of Imaging and Pathology, Faculty of Medicine, Biomedical Sciences Group, KU Leuven, 3000 Leuven, Belgium.
Feng ChenMarlein Miranda Cona, Yewei Liu, Ting Yin, Yuanbo Feng, Feng Chen, Stefaan Mulier, Raymond Oyen, Yicheng Ni, Department of Imaging and Pathology, Faculty of Medicine, Biomedical Sciences Group, KU Leuven, 3000 Leuven, Belgium.
Stefaan MulierMarlein Miranda Cona, Yewei Liu, Ting Yin, Yuanbo Feng, Feng Chen, Stefaan Mulier, Raymond Oyen, Yicheng Ni, Department of Imaging and Pathology, Faculty of Medicine, Biomedical Sciences Group, KU Leuven, 3000 Leuven, Belgium.
Yue LiMarlein Miranda Cona, Yewei Liu, Ting Yin, Yuanbo Feng, Feng Chen, Stefaan Mulier, Raymond Oyen, Yicheng Ni, Department of Imaging and Pathology, Faculty of Medicine, Biomedical Sciences Group, KU Leuven, 3000 Leuven, Belgium.
Jian ZhangMarlein Miranda Cona, Yewei Liu, Ting Yin, Yuanbo Feng, Feng Chen, Stefaan Mulier, Raymond Oyen, Yicheng Ni, Department of Imaging and Pathology, Faculty of Medicine, Biomedical Sciences Group, KU Leuven, 3000 Leuven, Belgium.
Raymond OyenMarlein Miranda Cona, Yewei Liu, Ting Yin, Yuanbo Feng, Feng Chen, Stefaan Mulier, Raymond Oyen, Yicheng Ni, Department of Imaging and Pathology, Faculty of Medicine, Biomedical Sciences Group, KU Leuven, 3000 Leuven, Belgium.
Yicheng NiMarlein Miranda Cona, Yewei Liu, Ting Yin, Yuanbo Feng, Feng Chen, Stefaan Mulier, Raymond Oyen, Yicheng Ni, Department of Imaging and Pathology, Faculty of Medicine, Biomedical Sciences Group, KU Leuven, 3000 Leuven, Belgium.
KU Leuven · BECentre Hospitalier Interrégional Edith Cavell · BECreative Research Enterprises (United States) · USJiangsu Provincial Academy of Traditional Chinese Medicine · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo facilitate translational research on cholelithiasis, we have developed a rat model of human gallstones by exploiting the unique biliopancreatic features of this species.

methodsUnder anesthesia, 16 adult rats of equal genders underwent two times of abdominal surgery. First, their common bile duct (CBD) was ligated to cause cholestasis by total biliary obstruction (TBO). On day 0, 1, 3, 7, 14, 21 and 28 after TBO, magnetic resonance imaging (MRI) was conducted to monitor the dilatation of the CBD, and blood was sampled to analyze total serum bilirubin (TSB). Secondly, on day 30, the abdomen was re-opened and gallstone(s) collected from human patients were implanted in the dilated CBD as a virtual gallbladder (VGB), which was closed by suture ligation. This rat cholelithiasis model was examined by MRI, clinical observation, microcholangiography and histology.

resultsAll rats survived two laparotomies. After ligation, the CBD was dilated to a stable size of 4 to 30 mm in diameter on day 21-28, which became a VGB. The rats initially showed signs of jaundice that diminished over time, which paralleled with the evolving TSB levels from 0.6 ± 0.3 mg/dL before ligation, through a peak of 10.9 ± 1.9 mg/dL on day 14, until a nearly normalized value after day 28. The dilated CBD with thickened wall allowed an incision for implantation of human gallstones of 1-10 mm in diameter. The rat cholelithiasis was proven by in vivo MRI and postmortem microcholangiography and histomorphology.

conclusionA rat model cholelithiasis with human gallstones has been established, which proves feasible, safe, reliable, nontoxic and cost-effective. Given the gallstones of human origin, applications of this model may be of help in translational research such as optical detection and lysis of gallstones by systemic drug administration.

Indexed as

BilirubinCholelithiasisCholestasisCommon bile ductGallbladderGallstonesRat

Identifiers

PMID27376020
PMCPMC4921946
OpenAlexW2460880230

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.