Evidence map›Paper›PMID 27375935›Full record

ArticleBiomedical optics express2016

Astigmatic multifocus microscopy enables deep 3D super-resolved imaging.

Laura Oudjedi, Jean-Bernard Fiche, Sara Abrahamsson, Laurent Mazenq, Aurélie Lecestre, Pierre-François Calmon, Aline Cerf, Marcelo Nöllmann

Open access · goldAbstract read
In one paragraph

Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
5.5field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Computational multifocal microscopy.Biomedical optics express · 2018
    Article
  6. Review
  7. Article
  8. Review
  9. Angular reconstitution-based 3D reconstructions of nanomolecular structures from superresolution light-microscopy images.Proceedings of the National Academy of Sciences of the United States of America · 2017
    Article
  10. Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 2 countries.

Laura OudjediCentre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 Rue de Navacelles, 34090 Montpellier, France.
Jean-Bernard FicheCentre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 Rue de Navacelles, 34090 Montpellier, France.
Sara AbrahamssonLulu and Anthony Wang Laboratory of Neural Circuits and Behavior, The Rockefeller University, New York, NY 10065, USA.
Laurent MazenqCNRS, LAAS, 7 Avenue du Colonel Roche, F-31400 Toulouse, France; Université de Toulouse, LAAS, F-31031 Toulouse, France.
Aurélie LecestreCNRS, LAAS, 7 Avenue du Colonel Roche, F-31400 Toulouse, France; Université de Toulouse, LAAS, F-31031 Toulouse, France.
Pierre-François CalmonCNRS, LAAS, 7 Avenue du Colonel Roche, F-31400 Toulouse, France; Université de Toulouse, LAAS, F-31031 Toulouse, France.
Aline CerfCNRS, LAAS, 7 Avenue du Colonel Roche, F-31400 Toulouse, France; Université de Toulouse, LAAS, F-31031 Toulouse, France.
Marcelo NöllmannCentre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 Rue de Navacelles, 34090 Montpellier, France.
Laboratoire des Technologies de la Microélectronique · FRUniversité de Montpellier · FRCentre de Biologie Structurale · FRInstitut de Recherches en Technologies et Sciences pour le Vivant · FRRockefeller University · US

Funding

European Research Council 260787
6 · The paper itself

Abstract

We have developed a 3D super-resolution microscopy method that enables deep imaging in cells. This technique relies on the effective combination of multifocus microscopy and astigmatic 3D single-molecule localization microscopy. We describe the optical system and the fabrication process of its key element, the multifocus grating. Then, two strategies for localizing emitters with our imaging method are presented and compared with a previously described deep 3D localization algorithm. Finally, we demonstrate the performance of the method by imaging the nuclear envelope of eukaryotic cells reaching a depth of field of ~4µm.

Indexed as

(050.1380) Binary optics(050.1950) Diffraction gratings(100.6640) Superresolution(110.1080) Active or adaptive optics(180.2520) Fluorescence microscopy(180.6900) Three-dimensional microscopy

Identifiers

PMID27375935
PMCPMC4918573
OpenAlexW2354253112

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.