ArticleBiomicrofluidics2016
Enrichment of diluted cell populations from large sample volumes using 3D carbon-electrode dielectrophoresis.
Article in Biomicrofluidics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Evaluating carbon-electrode dielectrophoresis under the ASSURED criteria.Frontiers in medical technology · 2022Article
- Dynamically controlled dielectrophoresis using resonant tuning.Electrophoresis · 2021Article
- Dielectrophoresis Prototypic Polystyrene Particle Synchronization toward Alive Keratinocyte Cells for Rapid Chronic Wound Healing.Sensors (Basel, Switzerland) · 2021Article
- A Prominent Cell Manipulation Technique in BioMEMS: Dielectrophoresis.Micromachines · 2020Review
- Analytical Guidelines for Designing Curvature-Induced Dielectrophoretic Particle Manipulation Systems.Micromachines · 2020Article
- Facile template-free synthesis of multifunctional 3D cellular carbon from edible rice paper.RSC advances · 2020Article
- Characterization of the Dielectrophoretic Response of Different Candida Strains Using 3D Carbon Microelectrodes.Micromachines · 2020Article
- Quantitative Investigation for the Dielectrophoretic Effect of Fluorescent Dyes at Single-Cell Resolution.ACS omega · 2018Article
- Dielectrophoretic Separation of Live and Dead Monocytes Using 3D Carbon-Electrodes.Sensors (Basel, Switzerland) · 2017Article
- Fabrication challenges and perspectives on the use of carbon-electrode dielectrophoresis in sample preparation.IET nanobiotechnology · 2017Review
- Numerical Model of Streaming DEP for Stem Cell Sorting.Micromachines · 2016Article
- Preface to Special Topic: Selected Papers from the 2015 Annual Meeting of the AES Electrophoresis Society in Salt Lake City, Utah.Biomicrofluidics · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we report on an enrichment protocol using carbon electrode dielectrophoresis to isolate and purify a targeted cell population from sample volumes up to 4 ml. We aim at trapping, washing, and recovering an enriched cell fraction that will facilitate downstream analysis. We used an increasingly diluted sample of yeast, 10(6)-10(2) cells/ml, to demonstrate the isolation and enrichment of few cells at increasing flow rates. A maximum average enrichment of 154.2 ± 23.7 times was achieved when the sample flow rate was 10 μl/min and yeast cells were suspended in low electrically conductive media that maximizes dielectrophoresis trapping. A COMSOL Multiphysics model allowed for the comparison between experimental and simulation results. Discussion is conducted on the discrepancies between such results and how the model can be further improved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.