ArticleFrontiers in microbiology2016
Identification and Characterization of Novel Small RNAs in Rickettsia prowazekii.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- A targeted mutational strategy aiding in generating antisense RNA to knockdown theJournal of bacteriology · 2026Article
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- Hybrid capture RNA-seq defines temporal gene expression inbioRxiv : the preprint server for biology · 2026Article
- A Small Non-Coding RNA Mediates Transcript Stability and Expression of Cytochrome bd Ubiquinol Oxidase Subunit I inInternational journal of molecular sciences · 2023Article
- Comparative transcriptomic analysis of Rickettsia conorii during in vitro infection of human and tick host cells.BMC genomics · 2020Article
- Prevalence of small base-pairing RNAs derived from diverse genomic loci.Biochimica et biophysica acta. Gene regulatory mechanisms · 2020Review
- Recent research milestones in the pathogenesis of human rickettsioses and opportunities ahead.Future microbiology · 2020Review
- Pathogenesis of Rickettsial Diseases: Pathogenic and Immune Mechanisms of an Endotheliotropic Infection.Annual review of pathology · 2019Review
- New approaches in the systematics of rickettsiae.New microbes and new infections · 2018Article
- 6S RNA, a Global Regulator of Transcription.Microbiology spectrum · 2018Review
- Impact of Three Different Mutations in Ehrlichia chaffeensis in Altering the Global Gene Expression Patterns.Scientific reports · 2018Article
- Enhancer Associated Long Non-coding RNA Transcription and Gene Regulation in Experimental Models of Rickettsial Infection.Frontiers in immunology · 2018Article
- Transcriptional profiling of Rickettsia prowazekii coding and non-coding transcripts during in vitro host-pathogen and vector-pathogen interactions.Ticks and tick-borne diseases · 2017Article
- MicroRNA Signature of Human Microvascular Endothelium Infected with Rickettsia rickettsii.International journal of molecular sciences · 2017Article
- Multi-omics Analysis Sheds Light on the Evolution and the Intracellular Lifestyle Strategies of Spotted Fever GroupFrontiers in microbiology · 2017Article
- Small Regulatory RNAs of Rickettsia conorii.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Emerging evidence implicates a critically important role for bacterial small RNAs (sRNAs) as post-transcriptional regulators of physiology, metabolism, stress/adaptive responses, and virulence, but the roles of sRNAs in pathogenic Rickettsia species remain poorly understood. Here, we report on the identification of both novel and well-known bacterial sRNAs in Rickettsia prowazekii, known to cause epidemic typhus in humans. RNA sequencing of human microvascular endothelial cells (HMECs), the preferred targets during human rickettsioses, infected with R. prowazekii revealed the presence of 35 trans-acting and 23 cis-acting sRNAs, respectively. Of these, expression of two trans-acting (Rp_sR17 and Rp_sR60) and one cis-acting (Rp_sR47) novel sRNAs and four well-characterized bacterial sRNAs (RNaseP_bact_a, α-tmRNA, 4.5S RNA, 6S RNA) was further confirmed by Northern blot or RT-PCR analyses. The transcriptional start sites of five novel rickettsial sRNAs and 6S RNA were next determined using 5' RLM-RACE yielding evidence for their independent biogenesis in R. prowazekii. Finally, computational approaches were employed to determine the secondary structures and potential mRNA targets of novel sRNAs. Together, these results establish the presence and expression of sRNAs in R. prowazekii during host cell infection and suggest potential functional roles for these important post-transcriptional regulators in rickettsial biology and pathogenesis.
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