ArticleScientific reports2016
Nanoscale imaging of untreated mammalian cells in a medium with low radiation damage using scanning electron-assisted dielectric microscopy.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
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- Observation of biological and emulsion samples by newly developed three-dimensional impedance scanning electron microscopy.Computational and structural biotechnology journal · 2024Article
- Quantitative evaluation of particle-binder interactions in ceramic slurries via differential centrifugal sedimentation.Scientific reports · 2024Article
- Direct observation of the effects of chemical fixation in MNT-1 cells: A SE-ADM and Raman study.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A Novel Approach for Identifying Nanoplastics by Assessing Deformation Behavior with Scanning Electron Microscopy.Micromachines · 2023Article
- Structural analysis of melanosomes in living mammalian cells using scanning electron-assisted dielectric microscopy with deep neural network.Computational and structural biotechnology journal · 2023Article
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- Raman scattering enhancement of dielectric microspheres on silicon nitride film.Scientific reports · 2022Article
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- Scanning Electron-Assisted Dielectric Microscopy Reveals Autophagosome Formation by LC3 and ATG12 in Cultured Mammalian Cells.International journal of molecular sciences · 2021Article
- Nanoscale observation of PM2.5 incorporated into mammalian cells using scanning electron-assisted dielectric microscope.Scientific reports · 2021Article
- Osteoblastic lysosome plays a central role in mineralization.Science advances · 2019Article
- Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy.PloS one · 2019Article
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- Applying Pattern Recognition to High-Resolution Images to Determine Cellular Signaling Status.IEEE transactions on nanobioscience · 2017Article
- High-resolution imaging of living mammalian cells bound by nanobeads-connected antibodies in a medium using scanning electron-assisted dielectric microscopy.Scientific reports · 2017Article
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2 authors.
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Abstract
Imaging of untreated living cells in a medium at a nanometre-scale resolution under physiological conditions is a significant challenge. Scanning electron microscopy (SEM) is widely used to observe cells in various atmospheric holders or special equipment. However, untreated biological specimens in aqueous solution generally incur heavy radiation damage from the direct electron beam (EB); and these images exhibit very poor contrast. Therefore, a new method for generating high-contrast images of living cells under physiological conditions without radiation damage has been strongly desired. Here, we demonstrate the first nanoscale observation of living cultured mammalian cells using our newly developed scanning-electron assisted dielectric microscopy (SE-ADM) method with a culture dish holder. Using the difference in relative permittivity between water and specimens, our SE-ADM system aids in the visualisation of untreated biological samples in aqueous solution. In addition, specimens incurred only a low level of radiation damage because the tungsten (W)-coated silicon nitride (SiN) film absorbs irradiated electrons. Untreated cells and organelles are clearly visible in high-contrast and high-resolution images without staining and fixation. Furthermore, our method enables the detection of changes in organelle structures within cells via time-lapse imaging with minimal radiation damage.
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