ArticleScientific reports2016
VirusMapper: open-source nanoscale mapping of viral architecture through super-resolution microscopy.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Who cites it
30 citing papers in PubMed, 53 citations in OpenAlex.
- Resolving viral structural complexity by super-resolution microscopy.Archives of virology · 2024Review
- Resolving the differential distribution of structural proteins in baculovirus using single-molecule localization microscopy.The Journal of general virology · 2024Article
- The vaccinia chondroitin sulfate binding protein drives host membrane curvature to facilitate fusion.EMBO reports · 2024Article
- Particle fusion of super-resolution data reveals the unit structure of Nup96 in Nuclear Pore Complex.Scientific reports · 2023Article
- Dynamics and Patterning of 5-Hydroxytryptamine 2 Subtype Receptors in JC Polyomavirus Entry.Viruses · 2022Article
- Poxviruses package viral redox proteins in lateral bodies and modulate the host oxidative response.PLoS pathogens · 2022Article
- Mapping molecular complexes with super-resolution microscopy and single-particle analysis.Open biology · 2022Review
- Joint registration of multiple point clouds for fast particle fusion in localization microscopy.Bioinformatics (Oxford, England) · 2022Article
- Virus morphology: Insights from super-resolution fluorescence microscopy.Biochimica et biophysica acta. Molecular basis of disease · 2022Review
- Detecting structural heterogeneity in single-molecule localization microscopy data.Nature communications · 2021Article
- Review
- Towards a Quantitative Single Particle Characterization by Super Resolution Microscopy: From Virus Structures to Antivirals Design.Frontiers in bioengineering and biotechnology · 2021Review
- Machine Learning and Artificial Intelligence for the Prediction of Host-Pathogen Interactions: A Viral Case.Infection and drug resistance · 2021Review
- A Quantitative Live-Cell Superresolution Imaging Framework for Measuring the Mobility of Single Molecules at Sites of Virus Assembly.Pathogens (Basel, Switzerland) · 2020Article
- Nanoscale polarization of the entry fusion complex of vaccinia virus drives efficient fusion.Nature microbiology · 2019Article
- Article
- Article
- NanoJ: a high-performance open-source super-resolution microscopy toolbox.Journal of physics D: Applied physics · 2019Review
- Automating multimodal microscopy with NanoJ-Fluidics.Nature communications · 2019Article
- A Spotlight on Viruses-Application of Click Chemistry to Visualize Virus-Cell Interactions.Molecules (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The nanoscale molecular assembly of mammalian viruses during their infectious life cycle remains poorly understood. Their small dimensions, generally bellow the 300nm diffraction limit of light microscopes, has limited most imaging studies to electron microscopy. The recent development of super-resolution (SR) light microscopy now allows the visualisation of viral structures at resolutions of tens of nanometers. In addition, these techniques provide the added benefit of molecular specific labelling and the capacity to investigate viral structural dynamics using live-cell microscopy. However, there is a lack of robust analytical tools that allow for precise mapping of viral structure within the setting of infection. Here we present an open-source analytical framework that combines super-resolution imaging and naïve single-particle analysis to generate unbiased molecular models. This tool, VirusMapper, is a high-throughput, user-friendly, ImageJ-based software package allowing for automatic statistical mapping of conserved multi-molecular structures, such as viral substructures or intact viruses. We demonstrate the usability of VirusMapper by applying it to SIM and STED images of vaccinia virus in isolation and when engaged with host cells. VirusMapper allows for the generation of accurate, high-content, molecular specific virion models and detection of nanoscale changes in viral architecture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.