ArticleJournal of structural biology2017
Cellular electron cryo tomography and in situ sub-volume averaging reveal the context of microtubule-based processes.
Article in Journal of structural biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- A generalizable and targeted molecular biopsy approach for in situ cryogenic electron tomography of vitreous brain tissue.Cell reports methods · 2025Article
- Co-clustering of EphB6 and ephrinB1 in trans restrains cancer cell invasion.Communications biology · 2024Article
- Handling Difficult Cryo-ET Samples: A Study with Primary Neurons from Drosophila melanogaster.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2023Article
- Bringing Structure to Cell Biology with Cryo-Electron Tomography.Annual review of biophysics · 2023Review
- Plasma FIB milling for the determination of structures in situ.Nature communications · 2023Article
- Lumenal components of cytoplasmic microtubules.Biochemical Society transactions · 2022Review
- Imaging Cytoskeleton Components by Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Structural analysis of receptors and actin polarity in platelet protrusions.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- [Progress in filters for denoising cryo-electron microscopy images].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2021Article
- DNA origami signposts for identifying proteins on cell membranes by electron cryotomography.Cell · 2021Article
- In situ cryo-electron tomography reveals filamentous actin within the microtubule lumen.The Journal of cell biology · 2020Article
- Looking back and looking forward: contributions of electron microscopy to the structural cell biology of gametes and fertilization.Open biology · 2020Review
- Three-dimensional organization of the cytoskeleton: A cryo-electron tomography perspective.Protein science : a publication of the Protein Society · 2020Review
- Coarse-Grained Simulation of Mechanical Properties of Single Microtubules With Micrometer Length.Frontiers in molecular biosciences · 2020Article
- Advances in cryo-electron tomography and subtomogram averaging and classification.Current opinion in structural biology · 2019Review
- CryoEM-based hybrid modeling approaches for structure determination.Current opinion in microbiology · 2018Review
- Exploring an optimal wavelet-based filter for cryo-ET imaging.Scientific reports · 2018Article
- Characterization of an industry-grade CMOS camera well suited for single molecule localization microscopy - high performance super-resolution at low cost.Scientific reports · 2017Article
- The advent of structural biologyBiophysics reports · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Electron cryo-tomography (cryoET) is currently the only technique that allows the direct observation of proteins in their native cellular environment. Sub-volume averaging of electron tomograms offers a route to increase the signal-to-noise of repetitive biological structures, such improving the information content and interpretability of tomograms. We discuss the potential for sub-volume averaging in highlighting and investigating specific processes in situ, focusing on microtubule structure and viral infection. We show that (i) in situ sub-volume averaging from single tomograms can guide and complement segmentation of biological features, (ii) the in situ determination of the structure of individual viruses is possible as they infect a cell, and (iii) novel, transient processes can be imaged with high levels of detail.
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Registered trials
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