ArticleScientific reports2016
High-speed atomic force microscopy reveals strongly polarized movement of clostridial collagenase along collagen fibrils.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Collagenase-mediated extracellular matrix targeting for enhanced drug penetration and therapeutic efficacy in nanoscale delivery systems for cancer therapy.Journal of nanobiotechnology · 2025Pooled it
- Structural Mechanism of Receptor-Triggered MyD88 Oligomeric Assembly in Innate Immune Signaling.Nature communications · 2026Article
- Bacterial collagenase harnesses collagen geometry for processive cleavage.Nature communications · 2026Article
- Investigating the influence of mineral content changes on mechanical properties through ligament insertion.Frontiers in aging · 2025Article
- Physiological mechanical forces accelerate the degradation of bovine lung collagen fibers by bacterial collagenase.Scientific reports · 2024Article
- Real-time single-molecule observation of incipient collagen fibrillogenesis and remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- In Operando Imaging Electrostatic-Driven Disassembly and Reassembly of Collagen Nanostructures.ACS nano · 2024Article
- The action of microbial collagenases in dentinal matrix degradation in root caries and potential strategies for its management: a comprehensive state-of-the-art review.Journal of applied oral science : revista FOB · 2024Review
- Changes in the Biomechanical Properties of Corneal Stromal Lens after Collagen Crosslinking Induced by EDC-NHS.Journal of ophthalmology · 2024Article
- Mechanical fatigue testing in silico: Dynamic evolution of material properties of nanoscale biological particles.Acta biomaterialia · 2023Article
- RETRACTED: ThePolymers · 2023Article
- Systematic evaluation of three porcine-derived collagen membranes for guided bone regeneration.Biomaterials translational · 2023Article
- Multiscale Characterization of Type I Collagen Fibril Stress-Strain Behavior under Tensile Load: Analytical vs. MD Approaches.Bioengineering (Basel, Switzerland) · 2022Article
- Assessing Collagen D-Band Periodicity with Atomic Force Microscopy.Materials (Basel, Switzerland) · 2022Review
- High-Speed Nanomechanical Mapping of the Early Stages of Collagen Growth by Bimodal Force Microscopy.ACS nano · 2021Article
- High-Speed Atomic Force Microscopy Reveals the Structural Dynamics of the Amyloid-β and Amylin Aggregation Pathways.International journal of molecular sciences · 2020Review
- Tension in fibrils suppresses their enzymatic degradation - A molecular mechanism for 'use it or lose it'.Matrix biology : journal of the International Society for Matrix Biology · 2020Review
- Effect of collagenase-gelatinase ratio on the mechanical properties of a collagen fibril: a combined Monte Carlo-molecular dynamics study.Biomechanics and modeling in mechanobiology · 2019Article
- CaThe FEBS journal · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial collagenases involved in donor infection are widely applied in many fields due to their high activity and specificity; however, little is known regarding the mechanisms by which bacterial collagenases degrade insoluble collagen in host tissues. Using high-speed atomic force microscopy, we simultaneously visualized the hierarchical structure of collagen fibrils and the movement of a representative bacterial collagenase, Clostridium histolyticum type I collagenase (ColG), to determine the relationship between collagen structure and collagenase movement. Notably, ColG moved ~14.5 nm toward the collagen N terminus in ~3.8 s in a manner dependent on a catalytic zinc ion. While ColG was engaged, collagen molecules were not only degraded but also occasionally rearranged to thicken neighboring collagen fibrils. Importantly, we found a similarity of relationship between the enzyme-substrate interface structure and enzyme migration in collagen-collagenase and DNA-nuclease systems, which share a helical substrate structure, suggesting a common strategy in enzyme evolution.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.